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[111In]oxine labelling of polymorphonuclear leucocytes: doubts concerning elution and effects on cell behaviour.

作者信息

Sheehan N J, Brown K A, Camacho A, Dumonde D C

出版信息

Int J Nucl Med Biol. 1985;12(3):243-7. doi: 10.1016/0047-0740(85)90032-4.

DOI:10.1016/0047-0740(85)90032-4
PMID:3934095
Abstract

Polymorphonuclear leucocytes (PMN) from normal human subjects were labelled with [111In]oxine (20 muCi 10(8) cells). In the presence of 20% autologous serum (AS), dissociation of 111In from the cells resulted in mean losses of radioactivity of 13% at 3 h and 30% at 24 h. Adherence of 111In-labelled PMN to cultured porcine endothelial monolayers was increased by 40.7 +/- 31.6% after 60 min incubation in 20% AS at 37 degrees C when compared with unlabelled cells. Phagocytosis and intracellular killing of Candida albicans were unaltered by labelling. Elution of 111In from labelled PMN together with enhanced adhesiveness may have important implications for the study of PMN kinetics and the investigation of inflammatory disease.

摘要

相似文献

1
[111In]oxine labelling of polymorphonuclear leucocytes: doubts concerning elution and effects on cell behaviour.
Int J Nucl Med Biol. 1985;12(3):243-7. doi: 10.1016/0047-0740(85)90032-4.
2
Conversion of 111In-oxine to 111In trichloride by acid hydrolysis.通过酸水解将111铟-奥克辛转化为三氯化111铟。
Int J Rad Appl Instrum A. 1987;38(1):70. doi: 10.1016/0883-2889(87)90241-3.
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[In vitro and in vivo studies using 111 indium oxine, 111 indium oxine sulfate and 99m Tc oxine in erythrocyte labeling].[使用111铟氧喹啉、111铟硫酸氧喹啉和99m锝氧喹啉进行红细胞标记的体外和体内研究]
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Kinetics of indium-oxine labelled red cells in haemolytic anaemias.铟-8-羟基喹啉标记红细胞在溶血性贫血中的动力学
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A comparison of two methods of labelling autologous platelets with 111In-oxine in five different species.五种不同物种中用111铟-奥克辛标记自体血小板的两种方法的比较。
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Labelling of lymphocytes with indium 111 oxine: effect on cell surface phenotype and antibody-dependent cellular cytotoxicity.用铟111草酸盐标记淋巴细胞：对细胞表面表型和抗体依赖性细胞毒性的影响。
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The comparison of 8-hydroxyquinoline, tropolone, and acetylacetone as mediators in the labelling of polymorphonuclear leucocytes with indium-111: a functional study.8-羟基喹啉、托酚酮和乙酰丙酮作为用铟-111标记多形核白细胞的介质的比较：一项功能研究。
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An improved method for the quantification of the in vivo kinetics of a representative population of 111In-labelled human platelets.一种改进的用于定量分析¹¹¹铟标记的人血小板代表性群体体内动力学的方法。
Eur J Nucl Med. 1985;10(11-12):522-7. doi: 10.1007/BF00252745.

引用本文的文献

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Rheumatoid blood decreases the adherence of polymorphonuclear cells (PMNs) to cultured endothelium.类风湿血液会降低多形核白细胞（PMNs）对培养内皮细胞的黏附。
Ann Rheum Dis. 1987 Feb;46(2):98-103. doi: 10.1136/ard.46.2.98.