Mountford P J, Allsopp M J, Hall F M, Wells C P, Coakley A J
Eur J Nucl Med. 1985;10(7-8):304-7. doi: 10.1007/BF00251301.
A simple method is described for estimating the activities bound to leucocytes, erythrocytes, platelets and the free activity, as resulted from the preparation and labelling of leucocytes with 111In-oxine. Measurements are required only of 111In activity, suspension volumes, and platelet concentrations. The limitations of the method are discussed. When blood from a normal volunteer was labelled by an 111In-oxine manufacturer's recommended technique, the greatest proportion of activity was bound to leucocytes, and in addition, a significant proportion of the activity was found to be associated with platelets. If the number of centrifugations between sedimentation and labelling was reduced from two to one, the proportion of free activity increased at the expense of a reduction in leucocyte activity, but the platelet activity remained unchanged. The relative distribution of cell-bound and free activities was independent of the relative centrifugal force (85-450 g), and of the time (15-30 min) and the suspension volume (5-10 ml) used to incubate the cells with 111In-oxine.
本文描述了一种简单的方法，用于估算用111铟-奥克辛标记白细胞后，与白细胞、红细胞、血小板结合的活性以及游离活性。仅需测量111铟活性、悬浮液体积和血小板浓度。讨论了该方法的局限性。当按照111铟-奥克辛制造商推荐的技术标记正常志愿者的血液时，最大比例的活性与白细胞结合，此外，还发现相当一部分活性与血小板相关。如果沉降和标记之间的离心次数从两次减少到一次，游离活性的比例增加，白细胞活性降低，但血小板活性保持不变。细胞结合活性和游离活性的相对分布与相对离心力（85 - 450克）、时间（15 - 30分钟）以及用于使细胞与111铟-奥克辛孵育的悬浮液体积（5 - 10毫升）无关。
