School of Food and Bioengineering, Chengdu University, Chengdu 610106, China.
School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China.
Bioresour Technol. 2024 Dec;413:131542. doi: 10.1016/j.biortech.2024.131542. Epub 2024 Sep 27.
Phycoerythrin (C-PE) is a cyanobacterial phycobiliprotein with extensive applications. This work sought to investigate the effects of various light conditions on C-PE accumulation by thermophilic Leptothermofonsia sichuanensis and characterize its C-PE stability and purity. Accumulation of C-PE as the predominant phycobiliprotein was significantly affected by light regime and light colours, reaching the highest C-PE accumulation (21.92 mg/g) under blue light. Importantly, the results suggested the superior C-PE thermostability of Leptothermofonsia than the mesophilic counterparts and good pH stability at a range of 4 to 7. Additionally, C-PE indicated advantageous potential for preservation as revealed by photostability experiments. Moreover, sorbitol, sucrose, and NaCl can further stabilise C-PE at 60 °C, of which 10 % sorbitol is the most effective. The extraction process herein resulted in a C-PE purity of 2.68, much higher than the food grade. Collectively, this work demonstrates the Leptothermofonsia strain as a promising bioresource for thermostable C-PE production.
藻红蛋白（C-PE）是一种广泛应用于医学领域的蓝藻藻胆蛋白。本研究旨在探讨不同光照条件对嗜热鞘丝藻（Leptothermofonsia sichuanensis）积累 C-PE 的影响，并对其 C-PE 稳定性和纯度进行了表征。结果表明，C-PE 作为主要的藻胆蛋白，其积累量受到光照条件和光色的显著影响，在蓝光下达到最高的 C-PE 积累量（21.92mg/g）。重要的是，结果表明 Leptothermofonsia 的 C-PE 热稳定性优于中温菌，在 pH 值为 4 到 7 的范围内具有良好的 pH 稳定性。此外，光稳定性实验表明 C-PE 具有有利的保存潜力。此外，山梨醇、蔗糖和 NaCl 可在 60°C 下进一步稳定 C-PE，其中 10%的山梨醇效果最佳。本研究的提取工艺得到的 C-PE 纯度为 2.68，远高于食品级纯度。综上所述，本研究表明嗜热鞘丝藻菌株是生产热稳定 C-PE 的有前途的生物资源。
