miR-NPs-RVG 促进脊髓损伤修复：来自脊髓源性微血管内皮细胞的启示。
miR-NPs-RVG promote spinal cord injury repair: implications from spinal cord-derived microvascular endothelial cells.
机构信息
Tianjin Key Laboratory of Spine and Spinal Cord, Department of Orthopaedics, International Science and Technology Cooperation Base of Spinal Cord Injury, Tianjin Medical University General Hospital, Tianjin, 300052, People's Republic of China.
Department of Orthopaedics, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, People's Republic of China.
出版信息
J Nanobiotechnology. 2024 Sep 28;22(1):590. doi: 10.1186/s12951-024-02797-7.
BACKGROUND
Spinal cord injury (SCI) often leads to a loss of motor and sensory function. Axon regeneration and outgrowth are key events for functional recovery after spinal cord injury. Endogenous growth of axons is associated with a variety of factors. Inspired by the relationship between developing nerves and blood vessels, we believe spinal cord-derived microvascular endothelial cells (SCMECs) play an important role in axon growth.
RESULTS
We found SCMECs could promote axon growth when co-cultured with neurons in direct and indirect co-culture systems via downregulating the miR-323-5p expression of neurons. In rats with spinal cord injury, neuron-targeting nanoparticles were employed to regulate miR-323-5p expression in residual neurons and promote function recovery.
CONCLUSIONS
Our study suggests that SCMEC can promote axon outgrowth by downregulating miR-323-5p expression within neurons, and miR-323-5p could be selected as a potential target for spinal cord injury repair.
背景
脊髓损伤（SCI）常导致运动和感觉功能丧失。轴突再生和生长是脊髓损伤后功能恢复的关键事件。轴突的内源性生长与多种因素有关。受神经和血管发育之间关系的启发，我们认为脊髓来源的微血管内皮细胞（SCMEC）在轴突生长中发挥重要作用。
结果
我们发现，在直接和间接共培养系统中与神经元共培养时，SCMEC 可以通过下调神经元中的 miR-323-5p 表达来促进轴突生长。在脊髓损伤大鼠中，使用神经元靶向纳米颗粒调节残留神经元中的 miR-323-5p 表达，促进功能恢复。
结论
我们的研究表明，SCMEC 可以通过下调神经元内的 miR-323-5p 表达来促进轴突生长，并且 miR-323-5p 可以作为脊髓损伤修复的潜在靶点。
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