Department of Neurology, Quanzhou First Hospital Affiliated to Fujian Medical University, Quanzhou, 362000, China.
J Health Popul Nutr. 2024 Sep 28;43(1):155. doi: 10.1186/s41043-024-00639-8.
This cross-sectional study aims to analyze the differences in gut flora between patients with epilepsy with and without cognitive impairment and normal subjects.
One hundred patients with epilepsy who came to our hospital from 2020.12 to 2022.12 (epilepsy group) were selected, and another 100 family members of the patients were selected as the control group (control group). Patients with epilepsy were further classified by the MMSE scale into 62 patients with combined cognitive impairment (Yes group) and 38 patients without cognitive impairment (No group). Detection of gut flora in feces by 16 S rRNA high-throughput sequencing. Logistic regression was used to analyze risk factors for cognitive dysfunction in patients with epilepsy.
There were more significant differences in the structure and composition of the gut flora between patients in the epilepsy group and the control group, but no significant differences in diversity analysis (P > 0.05). Actinobacteriota, Faecalibacterium and Collinsella were significantly lower in the Yes group than in the No group (P < 0.05), and the Alpha diversity index was numerically slightly smaller than in the No group, with the PCoA analysis demonstrating a more dispersed situation in both groups. Five metabolic pathways, including glycolysis and heterolactic fermentation, were upregulated in the Yes group. LEfSe analysis showed that five groups of bacteria, including Coriobacteriaceae and Collinsella, were selected as marker species for the presence or absence of comorbid cognitive impairment. Of these, Collinsella, Oscillospirales, and Ruminococcaceae have a greater impact on epilepsy combined with cognitive impairment.
There was an imbalance in the gut flora of patients with epilepsy compared to healthy controls. The gut flora of patients with epilepsy with cognitive dysfunction differs significantly from that of patients without cognitive dysfunction. Collinsella, Oscillospirales, and Ruminococcaceae have a greater impact on epilepsy with cognitive dysfunction and can be used as an indicator for the observation of epilepsy with cognitive dysfunction.
本横断面研究旨在分析伴有和不伴有认知障碍的癫痫患者与正常受试者之间肠道菌群的差异。
选取 2020 年 12 月至 2022 年 12 月来我院的 100 例癫痫患者（癫痫组），另选取 100 例患者家属作为对照组（对照组）。根据 MMSE 量表，将癫痫患者进一步分为 62 例合并认知障碍患者（Yes 组）和 38 例无认知障碍患者（No 组）。采用 16S rRNA 高通量测序检测粪便中的肠道菌群。采用 logistic 回归分析癫痫患者认知功能障碍的危险因素。
癫痫组患者与对照组患者的肠道菌群结构和组成差异更显著，但多样性分析无显著差异（P>0.05）。Yes 组的放线菌门、粪杆菌属和柯林斯菌属明显低于 No 组（P<0.05），且 Alpha 多样性指数略小于 No 组，两组的 PCoA 分析显示出更为分散的情况。Yes 组中有 5 种代谢途径，包括糖酵解和异型乳酸发酵，上调。LEfSe 分析显示，包括柯里伯氏菌科和柯林斯菌属在内的 5 组细菌被选为是否存在伴发认知障碍的标记物种。其中，柯林斯菌属、螺旋菌目和真杆菌科对癫痫合并认知障碍的影响更大。
与健康对照组相比，癫痫患者的肠道菌群失衡。伴有认知功能障碍的癫痫患者的肠道菌群与无认知功能障碍的癫痫患者有显著差异。柯林斯菌属、螺旋菌目和真杆菌科对癫痫合并认知功能障碍的影响更大，可作为观察癫痫合并认知功能障碍的指标。
