School of Life Sciences, B. S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, India.
Department of Pharmacy, Indira Gandhi National Tribal University, Madhya Pradesh, India.
Asian Pac J Cancer Prev. 2024 Sep 1;25(9):3159-3172. doi: 10.31557/APJCP.2024.25.9.3159.
Breast cancer is a global health concern, with millions of cases reported annually worldwide, making it the most common cancer among women. In India, the incidence of breast cancer has been steadily rising, reflecting a growing public health challenge and hence in the development of new drug moieties. Toxicity analysis of such novel drug candidates play a critical role in drug development, ensuring the safety and efficacy of potential therapeutics. Animal models, especially mice and zebrafish in the recent days, have been extensively used for toxicity evaluation owing to their physiological and genetic similarities to humans. This study was hence conducted with an aim to assess the toxicity using animal models, particularly mice and zebrafish.
In this study, 3-(2-(3,4-dimethoxyphenyl)-2-oxoethylidene)indolin-2-one (RAJI) - a chemically synthesised novel drug, was assessed for its toxicological potential in both zebrafish and mice models highlighting its survival, hatching, locomotor, neuromotor, behavioural abnormalities in zebrafish model and haematological and biochemical abnormalities in mice model.
The results obtained emphasise that no significant damages were seen in both zebrafish (survival, hatching, locomotor, neuromotor, behavioural abnormalities) and mice (body weight, haematological and biochemical abnormalities) models when administered in low doses.
All results obtained signifies that RAJI poses no harmful effects in the model organisms until administered in higher concentrations, thereby emphasising the fact that RAJI is a safe drug.
乳腺癌是一个全球性的健康问题，全球每年报告数百万例病例，使其成为女性中最常见的癌症。在印度，乳腺癌的发病率一直在稳步上升，反映出一个日益严峻的公共卫生挑战，因此需要开发新的药物。对这些新型候选药物进行毒性分析在药物开发中起着至关重要的作用，确保了潜在治疗药物的安全性和有效性。动物模型，尤其是最近的小鼠和斑马鱼，由于其与人类在生理和遗传上的相似性，已被广泛用于毒性评估。因此，本研究旨在使用动物模型，特别是小鼠和斑马鱼来评估其毒性。
在这项研究中，3-(2-(3,4-二甲氧基苯基)-2-氧代乙基)茚满-2-酮（RAJI）-一种化学合成的新型药物，在斑马鱼和小鼠模型中评估其毒理学潜力，重点研究其在斑马鱼模型中的生存、孵化、运动、神经运动、行为异常以及在小鼠模型中的血液学和生物化学异常。
研究结果强调，RAJI 在低剂量给药时，在两种模型动物（斑马鱼：生存、孵化、运动、神经运动、行为异常；小鼠：体重、血液学和生物化学异常）中均未观察到明显的损伤。
所有获得的结果都表明，RAJI 在给予更高浓度之前，在模型生物中不会产生有害影响，从而强调了 RAJI 是一种安全药物的事实。
