Assistant lecturer of Oral and Maxillofacial Pathology, Faculty of Dentistry, Sphinx University, Assiut, Egypt.
Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Minia University, Al Minia, Egypt.
Asian Pac J Cancer Prev. 2024 Sep 1;25(9):3219-3228. doi: 10.31557/APJCP.2024.25.9.3219.
Oral squamous cell carcinoma is ranked as the predominant type of head and neck squamous cell carcinoma, comprising roughly 90% of all oral cancer cases. Natural products have proven to be highly valuable as complementary, or adjunctive in the treatment of cancer. Piperine, a natural compound derived from Piper nigrum, demonstrates anti-proliferative and anti-neoplastic effects across various types of cancer. This study focused on assessing the cytotoxic effect of piperine in conjunction with cisplatin within the OSCC cell line.
In this in-vitro study, cultured OSCC cells were divided into four groups: a control group (untreated), a group exposed solely to piperine, a group exposed solely to cisplatin, and a group receiving both piperine and cisplatin. Cell viability was determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay technique. Additionally, flow cytometric analysis was employed to examine cell cycle progression and apoptosis. Assessment of reactive oxygen species activity, morphological changes, and nuclear area factor measurements were carried out. Expression of the apoptotic regulator Bax was assessed through western blotting analysis.
Piperine has cytotoxic and apoptotic effects in a concentration-dependent manner. Piperine in combination with cisplatin exhibited a synergistic effect, resulting in more pronounced inhibition of cell viability in OSCC cells compared to using piperine and cisplatin alone. Piperine and cisplatin for 24 h induced apoptosis strongly by increasing Bax protein and ROS activity.
Combining piperine with cisplatin demonstrated a greater effectiveness in triggering apoptosis in OSCC cells compared to using cisplatin alone, allowing for a reduction.
口腔鳞状细胞癌是头颈部鳞状细胞癌的主要类型，约占所有口腔癌病例的 90%。天然产物已被证明在癌症的治疗中具有很高的价值，可以作为辅助或补充治疗。胡椒碱是从胡椒中提取的一种天然化合物，在各种类型的癌症中都表现出抗增殖和抗肿瘤的作用。本研究旨在评估胡椒碱与顺铂联合应用于口腔鳞状细胞癌细胞系的细胞毒性作用。
在这项体外研究中，培养的口腔鳞状细胞癌细胞被分为四组：对照组（未处理）、单独暴露于胡椒碱组、单独暴露于顺铂组和同时暴露于胡椒碱和顺铂组。通过 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）测定法来确定细胞活力。此外，还采用流式细胞术分析来检查细胞周期进程和细胞凋亡。进行活性氧（ROS）活性、形态变化和核面积因子测量的评估。通过蛋白质印迹分析来评估凋亡调节剂 Bax 的表达。
胡椒碱以浓度依赖的方式具有细胞毒性和促凋亡作用。胡椒碱与顺铂联合使用具有协同作用，与单独使用胡椒碱和顺铂相比，更能显著抑制口腔鳞状细胞癌细胞的活力。胡椒碱和顺铂联合使用 24 小时可通过增加 Bax 蛋白和 ROS 活性强烈诱导细胞凋亡。
与单独使用顺铂相比，胡椒碱与顺铂联合使用在触发口腔鳞状细胞癌细胞凋亡方面更有效，从而减少了顺铂的用量。
