School of Materials ＆ Chemical Engineering, Zhongyuan University of Technology, Zhengzhou 450007, PR China.
School of Materials ＆ Chemical Engineering, Zhongyuan University of Technology, Zhengzhou 450007, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Feb 5;326:125203. doi: 10.1016/j.saa.2024.125203. Epub 2024 Sep 23.
An easily synthesized reaction-based probe for the fluorescence detection of Hg ions using was reported. The designed probe exhibited "turn-on" fluorescence response towards Hg ions via deprotection of the thiocarbonate-protecting group to alcohol in the HEPES/EtOH=8:2 (v/v, 5 mM, pH 7.4). The investigation results of probe Bzp-1 properties for Hg detection indicated that probe Bzp-1 has satisfactory high selectivity and sensitivity. The detection limit of Bzp-1 for Hg was found to be 4.2 nM. The recognition mechanism of Bzp-1 for Hg was confirmed by ESI-MS. Moreover, the probe Bzp-1 has been successfully used to rapidly detect trace amounts of hazardous Hg ions in real samples such as tap water, seafood and soil with good recoveries and less the relative standard deviations. Moreover, the Bzp-1 can also be used for fluorescence imagining of Hg in living cells.
报道了一种易合成的基于反应的探针，用于荧光检测 Hg 离子。该设计的探针通过保护的硫代碳酸盐保护基的脱保护，在 HEPES/EtOH=8:2（v/v，5 mM，pH 7.4）中对醇表现出“开启”的荧光响应。对探针 Bzp-1 用于 Hg 检测的性质的研究结果表明，探针 Bzp-1 具有令人满意的高选择性和灵敏度。发现 Bzp-1 对 Hg 的检测限为 4.2 nM。通过 ESI-MS 证实了 Bzp-1 对 Hg 的识别机制。此外，该探针 Bzp-1 已成功用于快速检测自来水中、海鲜和土壤等实际样品中的痕量有害 Hg 离子，回收率好，相对标准偏差小。此外，Bzp-1 还可用于活细胞中 Hg 的荧光成像。
