Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan province 450046, China; School of Animal Science and Technology, Foshan University, Foshan, Guangdong province 528225, China.
Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan province 450046, China.
Comp Immunol Microbiol Infect Dis. 2024 Oct;113:102244. doi: 10.1016/j.cimid.2024.102244. Epub 2024 Sep 27.
Members of the genus Bocaparvovirus have a significant impact on human health and can infect a wide range of hosts, increasing the likelihood of crossing species barriers. Among the various mammalian hosts, rodents are widely recognized as important reservoirs for emerging and zoonotic viruses. However, despite recent reports of bocavirus infections in rodents, our current understanding of rat bocavirus (RBoV) genetic diversity and evolution is limited. In this study, rodent samples were collected from the urban areas of Guangzhou city, Southern China, to investigate the presence and genetic diversity of RBoV. Through PCR-based screening of 296 rodent spleens, 54 samples were determined to be positive for RBoV infection, and 12 nearly complete genome sequences of RBoV were recovered. Phylogenetic analysis revealed distinct lineages and sub-lineages of RBoV, and six recombination events with strong statistical support were identified, with five of these events involving sequences obtained from this study. These results highlight the genetic diversity of RBoV circulating in rodents in Guangzhou city and emphasize the importance of extensive surveillance to gain a better understanding of RBoV epidemiology, evolutionary characteristics, and potential for cross-species transmission.
属 Bocaparvovirus 的成员对人类健康有重大影响，可以感染广泛的宿主，增加跨越物种障碍的可能性。在各种哺乳动物宿主中，啮齿动物被广泛认为是新兴和人畜共患病毒的重要宿主。然而，尽管最近有报道称啮齿动物感染了 bocavirus，但我们对大鼠 bocavirus (RBoV) 遗传多样性和进化的了解有限。在这项研究中，从中国南方广州市的城市地区采集了啮齿动物样本，以调查 RBoV 的存在和遗传多样性。通过对 296 个鼠脾的基于 PCR 的筛选，确定 54 个样本为 RBoV 感染阳性，并回收了 12 个几乎完整的 RBoV 基因组序列。系统发育分析显示 RBoV 存在明显的谱系和亚谱系，鉴定出 6 个具有强烈统计学支持的重组事件，其中 5 个事件涉及本研究获得的序列。这些结果突出了广州城市啮齿动物中循环的 RBoV 的遗传多样性，并强调了广泛监测的重要性，以更好地了解 RBoV 的流行病学、进化特征和跨物种传播的潜力。
