Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou, China.
Transbound Emerg Dis. 2019 Jan;66(1):259-267. doi: 10.1111/tbed.13011. Epub 2018 Sep 17.
Bocaparvovirus infections of humans and both wild and domestic animals have been widely reported around the world. In this study, we detected and genetically characterized porcine bocavirus (PBoV) carried by murine rodents (Rattus norvegicus, Rattus tanezumi, and Rattus losea) and house shrews (Suncus murinus) in China. Between May 2015 and May 2017, 496 murine rodents and 23 house shrews were captured in four Chinese provinces. Nested polymerase chain reaction was used to investigate the prevalence of PBoV in throat swab, faecal and serum samples. A total of 7.5% (39/519) throat swab samples, 60.5% (309/511) faecal samples, and 22.9% (52/227) serum samples were PBoV-positive. The prevalence among R. norvegicus and R. tanezumi was higher than that among R. losea and house shrews. PBoV-positive samples were found in all four provinces. Phylogenetic analysis based on partial viral capsid protein 1/2 (VP1/VP2) showed that sequences obtained in this study formed a novel group (PBoV G4). In addition, five near full-length PBoV genomes (4,715-4,798 nt) were acquired. These genomes encoded two non-structural proteins, NS1 (1,908 nt in four genomes and 1,923 nt in the remaining genome) and NP1 (600 nt), and the structural proteins, VP1/VP2 (1,851 nt). Phylogenetic analysis showed that PBoV G4 is distinct from rodent, human, and other bocaviruses. In conclusion, PBoV G4 prevalence was high among two common murine rodents in China, and the pathogenecity of PBoV G4 need to be further clarified.
世界各地已广泛报道人类以及野生动物和家养动物的 Bocaparvovirus 感染。在本研究中，我们检测并遗传特征鉴定了在中国携带的鼠源性啮齿动物（Norway 大鼠、褐家鼠和白腹巨鼠）和猪鼻鼩（Suncus murinus）中的猪博卡病毒（PBoV）。在 2015 年 5 月至 2017 年 5 月期间，在中国的四个省份中捕获了 496 只鼠源性啮齿动物和 23 只猪鼻鼩。巢式聚合酶链反应用于调查 PBoV 在咽喉拭子、粪便和血清样本中的流行率。共有 7.5%（39/519）的咽喉拭子样本、60.5%（309/511）的粪便样本和 22.9%（52/227）的血清样本为 PBoV 阳性。Norway 大鼠和褐家鼠的阳性率高于白腹巨鼠和猪鼻鼩。在所有四个省份都发现了 PBoV 阳性样本。基于病毒衣壳蛋白 1/2（VP1/VP2）的部分序列的系统进化分析表明，本研究中获得的序列形成了一个新的组（PBoV G4）。此外，还获得了五个近全长 PBoV 基因组（4715-4798nt）。这些基因组编码了两种非结构蛋白，NS1（4 个基因组中的 1908nt 和剩余基因组中的 1923nt）和 NP1（600nt），以及结构蛋白 VP1/VP2（1851nt）。系统进化分析表明，PBoV G4 与鼠源性、人类和其他博卡病毒明显不同。总之，PBoV G4 在我国两种常见的鼠源性啮齿动物中流行率较高，需要进一步阐明 PBoV G4 的致病性。
