Goda T, Takase S
School of Food and Nutritional Sciences, University of Shizuoka, Japan.
J Nutr. 1994 Nov;124(11):2233-9. doi: 10.1093/jn/124.11.2233.
The role of carbohydrate and fat in diet-induced modifications of jejunal disaccharidase activities was evaluated with an isoenergic diet containing a nonmetabolizable sugar, alpha-methylglucoside. Rats previously fed a high fat, low starch diet or a high starch low fat diet were force-fed three times over 12 h isoenergic high fat diets with or without alpha-methylglucoside, or a low fat diet containing alpha-methylglucoside. Regardless of the previous diet fed, force-feeding the high fat, alpha-methylglucoside diet produced significantly greater sucrase and lactase activities in the upper jejunum than force-feeding the high fat diet without alpha-methylglucoside; comparable or only slightly greater sucrase and lactase activities were seen in the lower jejunum. The animals fed the low fat, alpha-methylglucoside diet exhibited significantly greater sucrase and lactase activities in the lower jejunum than did the rats fed the high fat, alpha-methylglucoside diet; a less marked difference (< 30%) was observed between these two groups for disaccharidase activities in the upper jejunum. The lower sucrase and lactase activities observed in the jejunum of animals force-fed the high fat diet after consuming the high starch, low fat diet were accompanied by greater trypsin activity in the lumen of the upper and lower jejunum, suggesting that proteolytic degradation of sucrase and lactase might be stimulated in rats fed the high fat diets. These results suggest that both dietary carbohydrate and dietary fat independently and by different mechanisms modulate jejunal disaccharidase activities.
采用含有不可代谢糖α-甲基葡萄糖苷的等能量饮食，评估碳水化合物和脂肪在饮食诱导空肠双糖酶活性改变中的作用。先前喂食高脂肪、低淀粉饮食或高淀粉、低脂肪饮食的大鼠，在12小时内被强制喂食三次等能量高脂肪饮食，分别添加或不添加α-甲基葡萄糖苷，或喂食含有α-甲基葡萄糖苷的低脂肪饮食。无论先前喂食何种饮食，强制喂食高脂肪、α-甲基葡萄糖苷饮食后，空肠上段的蔗糖酶和乳糖酶活性显著高于强制喂食不含α-甲基葡萄糖苷的高脂肪饮食；空肠下段的蔗糖酶和乳糖酶活性相当或仅略高。喂食低脂肪、α-甲基葡萄糖苷饮食的动物空肠下段的蔗糖酶和乳糖酶活性显著高于喂食高脂肪、α-甲基葡萄糖苷饮食的大鼠；两组空肠上段双糖酶活性的差异较小（<30%）。在食用高淀粉、低脂肪饮食后被强制喂食高脂肪饮食的动物空肠中观察到较低的蔗糖酶和乳糖酶活性，同时空肠上段和下段肠腔内的胰蛋白酶活性较高，这表明喂食高脂肪饮食的大鼠中蔗糖酶和乳糖酶的蛋白水解降解可能受到刺激。这些结果表明，饮食中的碳水化合物和脂肪均通过不同机制独立调节空肠双糖酶活性。
