Hill J O, Burrell R
Clin Exp Immunol. 1979 Nov;38(2):332-41.
In order to determine the influence of an antigen's physical properties on the development of cell-mediated immunity (CMI) in the lung following aerosol immunization, human serum albumin (HSA) was prepared in either a soluble or a particulate form, the latter being coupled to respirable, carboxylated latex beads. Antigen was administered via an aerosol to groups of guinea-pigs, twice weekly for up to 4 weeks. Additional groups of animals served as unexposed and unconjugated latex controls. Lymphoid cells for CMI assays were isolated from the lung by bronchopulmonary lavage and from blood for use in mitogen- and antigen-induced lymphocyte transformation assays, as well as indirect macrophage migration inhibition tests. Particulate HSA-exposed animals yielded the highest numbers of free lung cells containing predominantly macrophages, with up to 33% lymphocytes. These were followed by the latex control, soluble HSA and unexposed control groups, respectively. Only the animals exposed to particulate HSA had evidence of antigen reactivation in the lung cell populations as measured by lymphocyte stimulation assays. In contrast, a response to polyclonal mitogens was found only in animals exposed to antigen in a soluble form. Data from macrophage depletion experiments suggest that the antigenicity of inhaled antigens may be due to the types and numbers of cells responding to the stimulus, and the subsequent role the alveolar macrophage may play in the modulation of cellular immunity.
为了确定抗原的物理性质对气溶胶免疫后肺部细胞介导免疫（CMI）发育的影响，制备了可溶性或颗粒性的人血清白蛋白（HSA），后者与可吸入的羧化乳胶珠偶联。通过气溶胶将抗原给予豚鼠组，每周两次，持续长达4周。另外几组动物作为未暴露和未偶联乳胶的对照。用于CMI测定的淋巴细胞通过支气管肺灌洗从肺中分离出来，并从血液中分离出来用于丝裂原和抗原诱导的淋巴细胞转化试验以及间接巨噬细胞迁移抑制试验。暴露于颗粒性HSA的动物产生的游离肺细胞数量最多，主要是巨噬细胞，淋巴细胞比例高达33%。其次分别是乳胶对照组、可溶性HSA组和未暴露对照组。通过淋巴细胞刺激试验测定，只有暴露于颗粒性HSA的动物在肺细胞群体中有抗原再激活的证据。相反，仅在暴露于可溶性抗原的动物中发现对多克隆丝裂原的反应。巨噬细胞耗竭实验的数据表明，吸入抗原的抗原性可能归因于对刺激作出反应的细胞类型和数量，以及肺泡巨噬细胞随后在细胞免疫调节中可能发挥的作用。
