Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.
Department of Orthopedics, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China.
ACS Appl Mater Interfaces. 2024 Oct 9;16(40):53541-53554. doi: 10.1021/acsami.4c11901. Epub 2024 Sep 30.
Hydrogels have garnered tremendous attention for their applications in the repair of intervertebral disk (IVD) degeneration and postoperative IVD defects. However, it is still challenging to develop a hydrogel fulfilling the requirements for high mechanical properties, adhesive capability, biocompatibility, antibacterial properties, and anti-inflammatory performance. Herein, we report a multifunctional double-network (DN) hydrogel composed of physically cross-linked carboxymethyl chitosan (CMCS) and tannic acid (TA) networks as well as chemically cross-linked acrylamide (AM) networks, which integrates the properties of high strength, adhesion, biocompatibility, antimicrobial activity, and anti-inflammation for the repair of postoperative IVD defects. The treatment with CMCS/TA/PAM DN hydrogels can significantly decrease the levels of inflammatory cytokines and degeneration-related factors and upregulated collagen type II alpha 1. In addition, the hydrogels can effectively seal the annulus fibrosus defect, prevent nucleus pulposus degeneration, retain IVD height, and restore the biomechanical properties of the disc to some extent. This polyphenol-mediated DN hydrogel is promising for sealing IVD defects and preventing herniation after lumbar discectomy.
水凝胶因其在修复椎间盘退变和术后椎间盘缺损中的应用而受到广泛关注。然而，开发一种同时满足高强度、粘附性、生物相容性、抗菌性能和抗炎性能要求的水凝胶仍然具有挑战性。在此，我们报告了一种由物理交联的羧甲基壳聚糖（CMCS）和单宁酸（TA）网络以及化学交联的丙烯酰胺（AM）网络组成的多功能双网络（DN）水凝胶，它集成了高强度、粘附性、生物相容性、抗菌活性和抗炎性，可用于修复术后椎间盘缺损。CMCS/TA/PAM DN 水凝胶治疗可显著降低炎症细胞因子和退变相关因子的水平，并上调 II 型胶原α 1。此外，水凝胶还可以有效封闭纤维环缺损，防止髓核退变，保持椎间盘高度，并在一定程度上恢复椎间盘的生物力学性能。这种多酚介导的 DN 水凝胶有望用于密封椎间盘缺损并防止腰椎间盘切除术后椎间盘突出。
