Jiang Xiping, Xu Hui, Sun Xinyue, Yang Xuefan, Xia Yuxuan, Xue Wen, He Yaohua
Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
College of Biological Science and Medical Engineering, Donghua University, Shanghai, China.
Front Bioeng Biotechnol. 2025 Jul 22;13:1642818. doi: 10.3389/fbioe.2025.1642818. eCollection 2025.
The high rate of retear following rotator cuff repair is largely attributed to the absence of a fibrocartilage layer and limited bone regeneration capacity. We aim to evaluate a bioadhesive derived from decellularized porcine annulus fibrosus extracellular matrix, loaded with zeolitic imidazolate framework-8 (ZIF-8), and to promote rotator cuff tendon-bone healing.
Three adhesive formulations were developed: (1) silk fibroin/tannic acid (ST group), (2) ST combined with decellularized porcine annulus fibrosus extracellular matrix (ST/dECM group), and (3) ST/dECM supplemented with ZIF-8 (ST/dECM/ZIF-8 group). Optimal component ratios were determined using lap shear strength testing. The microstructure, Fourier transform infrared (FTIR) spectra, swelling behavior, and degradation properties of the materials were characterized. studies assessed the adhesives' effects on cytotoxicity, proliferation, and the chondrogenic and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs). A rat rotator cuff repair model was used to evaluate anti-inflammatory effects, fibrocartilage and bone regeneration, and biomechanical performance.
All adhesive formulations exhibited comparable tissue adhesion strength and biocompatibility. Both the ST/dECM and ST/dECM/ZIF-8 groups enhanced BMSC chondrogenic differentiation compared to the ST group, with the ST/dECM/ZIF-8 group showing superior osteogenic induction. , the ST/dECM/ZIF-8 hydrogel effectively reduced interfacial inflammation and promoted fibrocartilage and bone regeneration. Biomechanical testing demonstrated significantly higher ultimate load, tensile stress, and stiffness in all adhesive-treated groups compared to untreated controls.
The ST/dECM/ZIF-8 bioadhesive hydrogel promotes fibrocartilage and bone regeneration. These findings highlight its potential as a promising biomaterial-based strategy to enhance tendon-to-bone interface healing following rotator cuff repair.
肩袖修复术后再撕裂率较高，这在很大程度上归因于缺乏纤维软骨层和有限的骨再生能力。我们旨在评估一种源自脱细胞猪纤维环细胞外基质并负载沸石咪唑酯骨架-8（ZIF-8）的生物粘合剂，以促进肩袖肌腱-骨愈合。
开发了三种粘合剂配方：（1）丝素蛋白/单宁酸（ST组），（2）ST与脱细胞猪纤维环细胞外基质联合使用（ST/dECM组），以及（3）补充ZIF-8的ST/dECM（ST/dECM/ZIF-8组）。使用搭接剪切强度测试确定最佳成分比例。对材料的微观结构、傅里叶变换红外（FTIR）光谱、溶胀行为和降解特性进行了表征。研究评估了粘合剂对大鼠骨髓间充质干细胞（BMSC）的细胞毒性、增殖以及软骨生成和成骨分化的影响。使用大鼠肩袖修复模型评估抗炎作用、纤维软骨和骨再生以及生物力学性能。
所有粘合剂配方均表现出相当的组织粘附强度和生物相容性。与ST组相比，ST/dECM组和ST/dECM/ZIF-8组均增强了BMSC软骨生成分化，其中ST/dECM/ZIF-8组显示出更好的成骨诱导作用。此外，ST/dECM/ZIF-8水凝胶有效减轻了界面炎症并促进了纤维软骨和骨再生。生物力学测试表明，与未处理的对照组相比，所有粘合剂处理组的极限载荷、拉伸应力和刚度均显著更高。
ST/dECM/ZIF-8生物粘合剂水凝胶促进纤维软骨和骨再生。这些发现突出了其作为一种有前景的基于生物材料的策略来增强肩袖修复后肌腱-骨界面愈合的潜力。
