Sultan Sherif El Sayed, Albadi Mazen Anwar Abdullah, Alhassan Faris Salah, Elsharkawy Sherif Magdy
Department of Prosthetic Dental Sciences, College of Dentistry, Jouf University, Sakakah, Saudi Arabia.
Department of Fixed Prosthodontics, College of Dentistry, Tanta University, Tanta, Egypt.
J Pharm Bioallied Sci. 2024 Jul;16(Suppl 3):S2491-S2493. doi: 10.4103/jpbs.jpbs_334_24. Epub 2024 Jun 8.
The increasing utilization of chairside computer-aided design/computer-aided manufacturing (CAD/CAM) block materials in dentistry necessitates a comprehensive evaluation of their cytotoxicity to ensure patient safety. This study aimed to assess the cytotoxic effects of commonly used chairside CAD/CAM block materials for fabricating dental restorations.
Several chairside CAD/CAM block materials including zirconia, lithium disilicate, and resin-based composites were evaluated for cytotoxicity using an model. Human gingival fibroblasts were cultured and exposed to extracts from each material. Cytotoxicity was assessed using MTT assay after 24, 48, and 72 hours of exposure. Additionally, cell morphology and viability were examined using microscopy.
The MTT assay revealed varying degrees of cytotoxicity among the tested materials. Zirconia demonstrated the least cytotoxicity with cell viability of 85% ± 5%, 82% ± 4%, and 79% ± 6% at 24, 48, and 72 hours, respectively. Lithium disilicate exhibited moderate cytotoxicity with cell viability of 70% ± 6%, 65% ± 7%, and 60% ± 5% at the corresponding time points. Resin-based composites displayed the highest cytotoxicity with cell viability of 55% ± 8%, 45% ± 6%, and 40% ± 4% at 24, 48, and 72 hours, respectively. Microscopic examination revealed altered cell morphology and reduced cell viability in the presence of resin-based composites.
Chairside CAD/CAM block materials exhibit varying degrees of cytotoxicity, with zirconia demonstrating the least and resin-based composites showing the highest cytotoxic effects. These findings underscore the importance of considering cytotoxicity profiles when selecting CAD/CAM materials for dental restorations to ensure patient safety and biocompatibility.
牙科椅旁计算机辅助设计/计算机辅助制造（CAD/CAM）块状材料的使用日益增加，因此有必要对其细胞毒性进行全面评估，以确保患者安全。本研究旨在评估用于制作牙科修复体的常用椅旁CAD/CAM块状材料的细胞毒性。
使用一种模型评估了几种椅旁CAD/CAM块状材料（包括氧化锆、二硅酸锂和树脂基复合材料）的细胞毒性。培养人牙龈成纤维细胞，并将其暴露于每种材料的提取物中。在暴露24、48和72小时后，使用MTT法评估细胞毒性。此外，使用显微镜检查细胞形态和活力。
MTT法显示，受试材料之间存在不同程度的细胞毒性。氧化锆的细胞毒性最小，在24、48和72小时时的细胞活力分别为85%±5%、82%±4%和79%±6%。二硅酸锂表现出中等细胞毒性，在相应时间点的细胞活力分别为70%±6%、65%±7%和60%±5%。树脂基复合材料的细胞毒性最高，在24、48和72小时时的细胞活力分别为55%±8%、45%±6%和40%±4%。显微镜检查显示，在存在树脂基复合材料的情况下，细胞形态发生改变，细胞活力降低。
椅旁CAD/CAM块状材料表现出不同程度的细胞毒性，氧化锆的细胞毒性最小，树脂基复合材料的细胞毒性最高。这些发现强调了在选择用于牙科修复体的CAD/CAM材料时考虑细胞毒性概况的重要性，以确保患者安全和生物相容性。
