Pidamale Raghavendra, Chauhan Prem S, Singh Raghavendr, Imran M D, Prakash Roopan, Kuppusamy Kasthuripriya
Department of Pediatric Dentistry, KVG Dental College and Hospital, Kurunjibhag, Sullia, Karnataka, India.
Department of Pediatric and Preventive Dentistry, Sharda Dental Clinic, Plot No 58/1. O.u.t. Colony Sainikpuri, Secunderabad, Telengana, India.
J Pharm Bioallied Sci. 2024 Jul;16(Suppl 3):S2664-S2666. doi: 10.4103/jpbs.jpbs_363_24. Epub 2024 Jul 18.
Severe Early Childhood Caries (S-ECC) is a prevalent oral health issue among young children, often associated with specific caries-associated bacteria. This study investigates the impact of diet on the growth and activity of caries-associated bacteria in S-ECC.
Plaque samples of and species were procured from laboratory. The samples were exposed to different dietary conditions, including high-sugar, high-carbohydrate, and control diets. The growth kinetics of caries-associated bacteria, including and species, were monitored using quantitative polymerase chain reaction (qPCR) and colony-forming unit (CFU) assays.
Under high-sugar conditions, the growth of increased by 50% compared to the control group, as evidenced by qPCR analysis. species also exhibited a significant increase in CFU counts in response to high-carbohydrate diets, showing a 40% rise compared to controls.
This study highlights the influence of diet on the proliferation of caries-associated bacteria in severe early childhood caries. High-sugar and high-carbohydrate diets contribute to the growth and activity of and species, exacerbating the progression of dental caries in affected children.
重度幼儿龋(S-ECC)是幼儿中普遍存在的口腔健康问题,通常与特定的致龋细菌有关。本研究调查饮食对S-ECC中致龋细菌生长和活性的影响。
从实验室获取变形链球菌和远缘链球菌的菌斑样本。将样本暴露于不同饮食条件下,包括高糖、高碳水化合物和对照饮食。使用定量聚合酶链反应(qPCR)和菌落形成单位(CFU)测定法监测致龋细菌(包括变形链球菌和远缘链球菌)的生长动力学。
qPCR分析表明,在高糖条件下,变形链球菌的生长比对照组增加了50%。远缘链球菌在高碳水化合物饮食条件下CFU计数也显著增加,与对照组相比增加了40%。
本研究突出了饮食对重度幼儿龋中致龋细菌增殖的影响。高糖和高碳水化合物饮食促进了变形链球菌和远缘链球菌的生长和活性,加剧了患龋儿童龋齿的进展。