Yang Xiaoyan, Lv Wenbo, Yang Yong, Yang Juan, Zhang Haiqing, Xu Zucai
Department of Neurology Affiliated Hospital of Zunyi Medical University Zunyi China.
Division of Clinical Neuroscience Chiba University Center for Forensic Mental Health Chiba Japan.
Ibrain. 2023 Jan 11;10(3):356-365. doi: 10.1002/ibra.12088. eCollection 2024 Fall.
It was found the expression of progesterone receptor membrane component 2 (PGRMC2) in the histone of epileptic mice was lower than that of normal mice. In this study, we found by the immunofluorescence technique, PGRMC2 was expressed in both astrocytes and neurons of the mouse hippocampus. In addition, the seizure latency and seizure grade of mice in each group were observed after stereotactic injection of the PGRMC2 knockdown virus, PGRMC2 overexpression lentivirus, and related null virus into the hippocampus of mice. It was found that the seizure latency of mice in the PTZ + siPGRMC2 group was prolonged compared with the null virus group. The seizure latency was shortened in the PTZ + PGRMC2 group. The number of grade IV and above seizures in the PTZ + siPGRMC2 group was significantly reduced, while the number of grade IV and above seizures in the PTZ + PGRMC2 group was significantly increased. It was found that the nerve cells in the PTZ + siPGRMC2 group were still intact. In the PTZ + PGRMC2 group, the neural cells were damaged, the intercellular space was widened, and the number of cells was reduced. These findings support that PGRMC2 may be involved in epileptic seizures.
研究发现，癫痫小鼠组蛋白中孕激素受体膜组分2（PGRMC2）的表达低于正常小鼠。在本研究中，我们通过免疫荧光技术发现，PGRMC2在小鼠海马体的星形胶质细胞和神经元中均有表达。此外，将PGRMC2基因敲低病毒、PGRMC2过表达慢病毒及相关空病毒立体定向注射到小鼠海马体后，观察各组小鼠的癫痫发作潜伏期和发作等级。结果发现，与空病毒组相比，PTZ+siPGRMC2组小鼠的癫痫发作潜伏期延长。PTZ+PGRMC2组小鼠的癫痫发作潜伏期缩短。PTZ+siPGRMC2组IV级及以上癫痫发作次数显著减少，而PTZ+PGRMC2组IV级及以上癫痫发作次数显著增加。结果发现，PTZ+siPGRMC2组神经细胞仍完整。PTZ+PGRMC2组神经细胞受损，细胞间隙增宽，细胞数量减少。这些研究结果支持PGRMC2可能参与癫痫发作。
