Alhakim Farah, Laham Antoun, Hasian Jameela
Department of Pharmaceutics Faculty of Pharmacy Damascus University, Damascus, Syria.
Adv Pharmacol Pharm Sci. 2024 Sep 20;2024:7027281. doi: 10.1155/2024/7027281. eCollection 2024.
A safer alternative made of plant extracts is needed, as evidenced by the negative effects of using synthetic sunscreen. Antioxidant properties of plants with high phenolic content have been reported. The goal of this research was to ascertain the phenolic content and antioxidant characteristics of ethanolic extracts made from grape and olive leaves under various extraction settings. The extracts were subjected to both qualitative and quantitative analyses using HPLC. Soxhlet extraction with 80% ethanol (v/v) as the solvent produced a result that was satisfactory. Four components of grape leaf extract and one component of olive leaf extract were identified. Expressed as gallic acid equivalents (GAEs), the total phenolic content (TPC) of the samples, as determined by using the Folin-Ciocalteu's reagent, ranged from 38.39 to 72.78 mg/g dry extract of olive and 65.918-132.7 mg/g dry extract of grape. An ethanolic extract of Salmoni (grape leaves) had the highest TPC (132 mg GAE/g), while an ethanolic extract of Zaity (olive leaves) had the lowest (72.66 ± 0.46 GAE/g). The Folin-Ciocalteu method proved the existence of antioxidants in the plant. By scavenging free radicals such as DPPH (2,2-diphenyl-1-picrylhydrazyl), the antioxidant capacity of the plant extracts was determined. Next, the ethanolic extracts of various cultivars of grape and olive leaves were analyzed to determine their sun protection factor (SPF) value. It was 28.8 and 29.96 for grape leaf extract and olive leaf extract, respectively.
由于使用合成防晒霜会产生负面影响,因此需要一种更安全的由植物提取物制成的替代品。据报道,含高酚类物质的植物具有抗氧化特性。本研究的目的是确定在不同提取条件下,葡萄叶和橄榄叶乙醇提取物中的酚类含量和抗氧化特性。使用高效液相色谱法(HPLC)对提取物进行定性和定量分析。以80%乙醇(v/v)作为溶剂进行索氏提取,结果令人满意。鉴定出葡萄叶提取物的四种成分和橄榄叶提取物的一种成分。用福林-酚试剂测定,样品的总酚含量(TPC)以没食子酸当量(GAEs)表示,橄榄干提取物中TPC范围为38.39至72.78mg/g,葡萄干提取物中为65.918 - 132.7mg/g。Salmoni(葡萄叶)乙醇提取物的TPC最高(132mg GAE/g),而Zaity(橄榄叶)乙醇提取物的TPC最低(72.66±0.46 GAE/g)。福林-酚法证明了植物中存在抗氧化剂。通过清除2,2-二苯基-1-苦基肼(DPPH)等自由基来测定植物提取物的抗氧化能力。接下来,分析了不同品种葡萄叶和橄榄叶的乙醇提取物,以确定它们的防晒系数(SPF)值。葡萄叶提取物和橄榄叶提取物的SPF值分别为28.8和29.96。