源自人脱落乳牙干细胞的因子可逆转帕金森病斑马鱼模型中的神经功能缺损。
Factors derived from human exfoliated deciduous teeth stem cells reverse neurological deficits in a zebrafish model of Parkinson's disease.
作者信息
Chen Yong-Ren, Wong Chin-Chean, Chen Yi-No, Yang Bing-Heng, Lee Po-Hui, Shiau Chia-Yang, Wang Kuo-Chuan, Li Chung-Hsing
机构信息
Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
出版信息
J Dent Sci. 2024 Oct;19(4):2035-2044. doi: 10.1016/j.jds.2024.06.004. Epub 2024 Jun 17.
BACKGROUND/PURPOSE: Mesenchymal stem cells exhibit therapeutic efficacy for brain injury. This study examined the effect of mesenchymal stem cells derived from human exfoliated deciduous teeth (SHED) on alleviating symptoms of Parkinson's disease (PD).
MATERIALS AND METHODS
SHED were isolated to examine the biosafety and bioavailability of stem cells derived from human exfoliated deciduous teeth-derived conditioned medium (SHED-CM) for the alleviation of PD symptoms in a 6-hydroxydopamine (6-OHDA)-induced PD zebrafish model.
RESULTS
SHED-CM administration did not induce neurological, skin or muscle toxicity in control zebrafish at any dose, and estrogen equivalent testing showed no chronic toxicants. Induction of PD with 6-OHDA suppressed zebra SHED-CM was administered to zebrafish treated with 6-OHDA to induce PD symptoms. Similar to nomifensine, a drug with proven anti-PD potential, SHED-CM repaired the motor deficiencies in the zebrafish PD model.
CONCLUSION
Our results indicate the biosafety of SHED-CM and its therapeutic potential in treating PD in a zebrafish model.
背景/目的：间充质干细胞对脑损伤具有治疗效果。本研究检测了源自人脱落乳牙的间充质干细胞（SHED）对减轻帕金森病（PD）症状的作用。
材料与方法
分离SHED，检测源自人脱落乳牙的条件培养基（SHED-CM）中干细胞的生物安全性和生物利用度，以减轻6-羟基多巴胺（6-OHDA）诱导的PD斑马鱼模型中的PD症状。
结果
在任何剂量下，给予SHED-CM均未在对照斑马鱼中诱导神经、皮肤或肌肉毒性，雌激素等效性测试显示无慢性毒物。用6-OHDA诱导PD可抑制斑马鱼，将SHED-CM给予用6-OHDA处理以诱导PD症状的斑马鱼。与具有已证实的抗PD潜力的药物诺米芬辛类似，SHED-CM修复了斑马鱼PD模型中的运动缺陷。
结论
我们的结果表明SHED-CM的生物安全性及其在斑马鱼模型中治疗PD的潜力。
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