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水泥细胞衍生的细胞外囊泡调节破骨细胞生成和成骨细胞生成。

Cementocyte-derived extracellular vesicles regulate osteoclastogenesis and osteoblastogenesis.

作者信息

Li Jiajun, Sakisaka Yukihiko, Nemoto Eiji, Maruyama Kentaro, Suzuki Shigeki, Xiong Kaixin, Tada Hiroyuki, Tenkumo Taichi, Yamada Satoru

机构信息

Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan.

Division of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai, Japan.

出版信息

J Dent Sci. 2024 Oct;19(4):2236-2246. doi: 10.1016/j.jds.2024.02.025. Epub 2024 Mar 2.

DOI:10.1016/j.jds.2024.02.025
PMID:39347082
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11437308/
Abstract

BACKGROUND/PURPOSE: Cementum shares many properties with bone; however, in contrast to bone, it is not innervated or vascularized and has a limited capacity for remodeling. Osteocytes located in the lacunae-canalicular system of bone tissue play a central role in bone remodeling by communicating with osteoblasts and osteoclasts. Although cementocytes are present in cellular cementum and are morphologically similar to osteocytes, it remains unclear whether they are involved in the dynamic functional regulation of metabolism in cementum. The present study focused on the extracellular vesicles (EVs) secreted by cementocytes and examined their effects on osteoclasts and osteoblasts.

MATERIALS AND METHODS

EVs were extracted from the mouse cementocyte cell line, IDG-CM6. The effects of EVs on recombinant RANKL-induced osteoclastogenesis and recombinant Bone morphogenetic protein (BMP)-2-mediated osteoblastogenesis were investigated using the mouse osteoclast progenitor cell line, RAW264.7 and mouse pre-osteoblast cell line, MC3T3-E1, respectively.

RESULTS

EVs enhanced the formation of tartrate-resistant acid phosphatase activity-positive cells. Real-time PCR revealed that EVs up-regulated the expression of osteoclast-related genes. On the other hand, the cell culture supernatant of cementocytes significantly inhibited the differentiation of osteoclasts. Regarding osteoblastogenesis, EVs suppressed the expression of alkaline phosphatase, bone sialoprotein, and osteocalcin induced by recombinant BMP-2 at the gene and protein levels.

CONCLUSION

A network of cementocytes, osteoblasts, and osteoclasts may exist in cellular cementum, which suggests the involvement of cementocytes in dynamic metabolism of cementum through EVs.

摘要

背景/目的：牙骨质与骨有许多共同特性；然而，与骨不同的是，牙骨质没有神经支配和血管化，且重塑能力有限。位于骨组织陷窝-小管系统中的骨细胞通过与成骨细胞和破骨细胞通讯在骨重塑中起核心作用。尽管牙骨质细胞存在于细胞性牙骨质中且在形态上与骨细胞相似，但它们是否参与牙骨质代谢的动态功能调节仍不清楚。本研究聚焦于牙骨质细胞分泌的细胞外囊泡（EVs），并研究了它们对破骨细胞和成骨细胞的影响。

材料与方法

从小鼠牙骨质细胞系IDG-CM6中提取EVs。分别使用小鼠破骨细胞前体细胞系RAW264.7和小鼠前成骨细胞系MC3T3-E1研究了EVs对重组核因子κB受体活化因子配体（RANKL）诱导的破骨细胞生成和重组骨形态发生蛋白（BMP）-2介导的成骨细胞生成的影响。

结果

EVs增强了抗酒石酸酸性磷酸酶活性阳性细胞的形成。实时聚合酶链反应（PCR）显示EVs上调了破骨细胞相关基因的表达。另一方面，牙骨质细胞的细胞培养上清液显著抑制破骨细胞的分化。关于成骨细胞生成，EVs在基因和蛋白水平上抑制了重组BMP-2诱导的碱性磷酸酶、骨唾液蛋白和骨钙素的表达。

结论

细胞性牙骨质中可能存在牙骨质细胞、成骨细胞和破骨细胞网络，这表明牙骨质细胞通过EVs参与牙骨质的动态代谢。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/2b0e083df877/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/0d98e155803a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/95d15bc28940/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/c6a216e5bae6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/f9c59ab768a9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/88562ae048e0/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/35c55496e134/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/9d497dcb2c04/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/2b0e083df877/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/0d98e155803a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/95d15bc28940/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/c6a216e5bae6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/f9c59ab768a9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/88562ae048e0/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/35c55496e134/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/9d497dcb2c04/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8988/11437308/2b0e083df877/gr8.jpg

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Exosomes from TNF-α-treated human gingiva-derived MSCs enhance M2 macrophage polarization and inhibit periodontal bone loss.来自肿瘤坏死因子-α处理的人牙龈间充质干细胞的外泌体可增强M2巨噬细胞极化并抑制牙周骨丢失。
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