Kumari Sangeeta, Singh Kalpana, Kumar Abhigyan, Kumari Shubhanti, Raina Aditi
Reproductive Medicine, Indira Gandhi Institute of Medical Sciences (IGIMS), Patna, IND.
General Medicine, Netaji Subhas Medical College and Hospital, Patna, IND.
Cureus. 2024 Aug 30;16(8):e68209. doi: 10.7759/cureus.68209. eCollection 2024 Aug.
Introduction Semen quality, characterized by parameters such as sperm count, motility, and morphology, determines successful fertilization and subsequent embryo health. This study investigates the impact of sperm parameters on embryo quality in assisted reproductive technology (ART). Methods The study utilized a retrospective design with 194 male and 194 female participants who underwent fertility treatment from October 2020 to May 2024. Semen analysis included assessment of sperm count, motility, and morphology. Embryo quality was evaluated on days three and five post-fertilizations based on morphological criteria. Statistical analyses, including one-way ANOVA and chi-square tests, explored relationships between semen parameters and embryo quality. Results The study included 388 participants (males: 194 and females: 194). Female participants had a mean age of 31.0 ± 4.6 years and a mean BMI of 23.1 ± 5.3 kg/m², while males had a mean age of 36.6 ± 5.4 years and a mean BMI of 22.7 ± 2.8 kg/m². Paternal age and BMI showed no significant association (p > 0.05) with embryo quality. However, sperm quality parameters such as sperm count, motility, and morphology demonstrated significant associations (p < 0.05) with embryo quality on both day three and day five, indicating that abnormal sperm parameters were linked to poorer embryo quality. Factors such as alcohol consumption, smoking, tobacco use, living in industrial areas, and tea/coffee consumption showed no significant association with embryo quality. Conclusion The findings of the study emphasize the importance of comprehensive semen analysis in fertility assessments and highlight opportunities for improving ART outcomes through targeted interventions and further mechanistic research.
引言 精液质量由精子数量、活力和形态等参数表征，决定着受精的成功与否以及后续胚胎的健康状况。本研究调查了辅助生殖技术（ART）中精子参数对胚胎质量的影响。
方法 本研究采用回顾性设计，纳入了2020年10月至2024年5月期间接受生育治疗的194名男性和194名女性参与者。精液分析包括对精子数量、活力和形态的评估。基于形态学标准，在受精后的第三天和第五天对胚胎质量进行评估。包括单因素方差分析和卡方检验在内的统计分析探索了精液参数与胚胎质量之间的关系。
结果 该研究包括388名参与者（男性194名，女性194名）。女性参与者的平均年龄为31.0±4.6岁，平均体重指数为23.1±5.3kg/m²，而男性的平均年龄为36.6±5.4岁，平均体重指数为22.7±2.8kg/m²。父亲的年龄和体重指数与胚胎质量无显著关联（p>0.05）。然而，精子数量、活力和形态等精子质量参数在第三天和第五天都与胚胎质量表现出显著关联（p<0.05），表明异常的精子参数与较差的胚胎质量相关。饮酒、吸烟、使用烟草、居住在工业区以及饮用茶/咖啡等因素与胚胎质量无显著关联。
结论 该研究结果强调了在生育评估中进行全面精液分析的重要性，并突出了通过有针对性的干预措施和进一步的机制研究来改善辅助生殖技术结果的机会。
