Gansu Research Academy of Forestry Science and Technology, Lanzhou, Gansu Province, 730020, People's Republic of China.
College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu Province, 730070, People's Republic of China.
Plant Cell Rep. 2024 Sep 30;43(10):247. doi: 10.1007/s00299-024-03323-7.
High-throughput next-generation sequencing of 161 olive germplas. 33 samples were selected as core olive germplasm and Fingerprints were constructed. After GWAS analysis of olive leaf shape, 14 candidate genes were localized. Olive (Olea europaea L.) has been introduced to China since the 1960s. After a prolonged period of variation and domestication, there is a lack of comprehensive research on its genetics. The olive oil directly extracted from Olea europaea L. is recognized as 'liquid gold', nevertheless, people constantly overlook the valuable wealth of olive leaves. High-throughput next-generation sequencing was performed on 161 olive germplasm to analyze the kinship, genetic structure and diversity of olives, and the core germplasm of olives were selected and fingerprints were constructed. Meanwhile, Genome-wide association analysis (GWAS) was performed to locate the gene for regulating olive leaf shape. Herein, the results parsed that most of the Chinese olive germplasm was more closely related to the Italian germplasm. A wealth of hybridized germplasm possessed high genetic diversity and had the potential to be used as superior parental material for olive germplasm. A total of 33 samples were selected and characterized as core germplasm of olive and Fingerprints were also constructed. A total of 14 candidate genes were localized after GWAS analysis of four olive leaf shape phenotypes, including leaf shape, leaf curvature shape, leaf tip and leaf base shape. Collectively, this study revealed the genetic basis of olives in China and also succeeded in constructing the core germplasm that stands for the genetic diversity of olives, which can contribute to the scientific and effective collection and preservation of olive germplasm resources, and provide a scientific basis for the in-depth excavation and utilization of genes regulating olive leaf shape.
高通量下一代测序的 161 个油橄榄种质。选择 33 个样本作为核心油橄榄种质，并构建指纹图谱。对橄榄叶形进行 GWAS 分析后，定位到 14 个候选基因。橄榄（Olea europaea L.）自 20 世纪 60 年代引入中国。经过长期的变异和驯化，其遗传学缺乏全面研究。直接从 Olea europaea L. 中提取的橄榄油被称为“液体黄金”，然而，人们却常常忽略了橄榄叶的宝贵财富。对 161 个油橄榄种质进行高通量下一代测序，分析油橄榄的亲缘关系、遗传结构和多样性，选择油橄榄核心种质并构建指纹图谱。同时，进行全基因组关联分析（GWAS）定位调控橄榄叶形的基因。结果表明，中国的油橄榄种质与意大利的种质关系最为密切。大量杂交种质具有丰富的遗传多样性，有潜力作为油橄榄种质的优良亲本材料。共选择 33 个样本作为油橄榄核心种质进行特征描述，并构建指纹图谱。对四种橄榄叶形表型（叶形、叶卷曲形、叶尖和叶基形状）进行 GWAS 分析后，共定位到 14 个候选基因。综上所述，本研究揭示了中国油橄榄的遗传基础，成功构建了代表油橄榄遗传多样性的核心种质，有助于科学有效地收集和保存油橄榄种质资源，为深入挖掘和利用调控橄榄叶形的基因提供了科学依据。
