Seino J, Fukuda K, Kinoshita Y, Sudo K, Horigome I, Sato H, Saito T, Furuyama T, Yoshinaga K
Tohoku J Exp Med. 1985 Sep;147(1):111-2. doi: 10.1620/tjem.147.111.
C3 nephritic factor (C3 NeF) was measured by assessing its capacity to form complex with C3 and B using an enzyme-linked immunosorbent assay (ELISA). Incubation of C3 NeF with normal human serum in the presence of MgEGTA resulted in a dose-dependent increase of C3-B-IgG complex. No complex was formed in EDTA. The C3 NeF titer estimated in this way was in good accordance with those reported previously by other indirect methods.
通过酶联免疫吸附测定（ELISA）评估其与C3和B形成复合物的能力来检测C3肾炎因子（C3 NeF）。在MgEGTA存在的情况下，将C3 NeF与正常人血清孵育会导致C3-B-IgG复合物剂量依赖性增加。在EDTA中未形成复合物。以这种方式估计的C3 NeF滴度与其他间接方法先前报道的结果高度一致。
