Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
Methods Mol Biol. 2025;2857:99-107. doi: 10.1007/978-1-0716-4128-6_9.
One of the characteristics of aging and age-related disorders is the formation and evolution of a chronic, low-grade, and hence subclinical, inflammatory state known as inflammaging. Although the progression of inflammaging is now recognized as one of the main driving forces of aging and one of the main risk factors for morbidity and mortality in older subjects, current knowledge on the causative agents of inflammaging itself and chronic, aging-related diseases is still incomplete. In this chapter, we offer a methodological approach for assessing inflammation associated with aging through the use of multiplex immunoassay, which enables the rapid, reproducible, and simultaneous dosage of several cytokines, chemokines, and inflammatory mediators with little biological sample usage.
衰老和与年龄相关的疾病的特征之一是形成和发展一种慢性、低度、因此是亚临床的炎症状态，称为炎老化。尽管炎老化的进展现在被认为是衰老的主要驱动力之一，也是老年患者发病率和死亡率的主要危险因素之一，但关于炎老化本身和慢性、与衰老相关的疾病的致病因素的现有知识仍然不完整。在本章中，我们通过使用多重免疫测定法提供了一种评估与衰老相关的炎症的方法学方法，该方法能够快速、可重复且同时测定少量生物样本中几种细胞因子、趋化因子和炎症介质的剂量。
