Multiscale Research Institute for Complex Systems, Fudan University, Shanghai, China.
Methods Mol Biol. 2025;2857:109-115. doi: 10.1007/978-1-0716-4128-6_10.
The aging immune system undergoes significant changes, leading to a state known as immunosenescence. Understanding the molecular mechanisms underlying immunosenescence is crucial for developing targeted interventions to enhance immune functions in older individuals. This bio-protocol review focuses on the application of quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for the mRNA quantification of cytokine-inducible SH2-containing protein (CISH), an immune regulator overexpressed in T-cell responses from older adults. We outline a comprehensive protocol for the quantitative assessment of CISH mRNA expression, providing a valuable tool for researchers investigating immunosenescence.
衰老的免疫系统会发生重大变化，导致一种称为免疫衰老的状态。了解免疫衰老的分子机制对于开发针对老年人增强免疫功能的靶向干预措施至关重要。本生物议定书综述重点介绍了定量逆转录聚合酶链反应 (qRT-PCR) 在细胞因子诱导的含 SH2 结构域蛋白 (CISH) mRNA 定量中的应用，CISH 是一种在老年人 T 细胞反应中过度表达的免疫调节剂。我们概述了定量评估 CISH mRNA 表达的综合方案，为研究免疫衰老的研究人员提供了有价值的工具。
