Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi, Pathum Thani 12110, Thailand.
Faculty of Science and Technology, Princess of Naradhiwas University, Narathiwat 96000, Thailand.
Food Chem. 2025 Jan 15;463(Pt 4):141427. doi: 10.1016/j.foodchem.2024.141427. Epub 2024 Sep 24.
A sensitive dual immunochromatographic test strip (dual-ICTS) was developed to detect malachite green (MG) and its metabolite, leucomalachite green (LMG), using two types of gold nanoparticles: round-shaped (red) and star-shaped (blue). The detection limits were determined to be 0.221 μg L for MG and 0.214 μg L for LMG, respectively. The dual-ICTS provided a cut-off value of 1.8 μg L for MG and LMG detection. The dual-ICTS successfully detected MG and LMG in food samples, with recovery rates ranging from 86 % to 116 %. The dual-ICTS was evaluated by correlation analysis between the proposed assay and the well-established enzyme-linked immunosorbent assay in the MG and LMG detection. This is the first report on the development of the ICTS that can detect both MG and LMG at the same time within only 5 min, making it a sensitive and rapid tool for on-site detection.
研制出一种灵敏的双免疫层析试纸条（dual-ICTS），用于同时检测孔雀石绿（MG）及其代谢物隐色孔雀石绿（LMG），该试纸条使用两种类型的金纳米粒子：圆形（红色）和星形（蓝色）。MG 和 LMG 的检测限分别为 0.221μg/L 和 0.214μg/L。双免疫层析试纸条为 MG 和 LMG 的检测提供了 1.8μg/L 的截断值。该双免疫层析试纸条成功地检测了食品样品中的 MG 和 LMG，回收率在 86%至 116%之间。通过在 MG 和 LMG 检测中，将该检测方法与已建立的酶联免疫吸附测定法进行相关性分析，对双免疫层析试纸条进行了评估。这是首次报道能够在 5 分钟内同时检测 MG 和 LMG 的 ICTS，使其成为一种灵敏、快速的现场检测工具。
