Department of Breast Surgery and Oncology, Tokyo Medical University, Tokyo, Japan.
Department of Pathology (Medical Research Center), Institute of Medical Science, Tokyo Medical University, Tokyo, Japan;
Anticancer Res. 2024 Oct;44(10):4525-4536. doi: 10.21873/anticanres.17281.
BACKGROUND/AIM: Patients with triple-negative breast cancer (TNBC) who obtain a pathological complete response (pCR) after neoadjuvant chemotherapy have an improved prognosis. Lymphocyte-predominant breast cancer is more likely to respond to neoadjuvant chemotherapy. Here, we investigated the correlation between tumor-infiltrating lymphocytes (TILs) in pre-treatment biopsy specimens from patients with TNBC in relation to response to NAC.
The level of infiltration by immune cells expressing immune cell lineage surface markers (CD8, CD4, CD19, CD14, CD11c, and CD11b) in biopsy specimens from 52 patients with TNBC was examined using multispectral immunofluorescent labelling.
The level of CD8-positive TILs was significantly higher in patients with a pCR (p=0.045). The Cox proportional hazard model confirmed that lymph node involvement was associated with poorer disease-free survival (p=0.008). A high level of CD8-positive TILs was related to significantly prolonged disease-free survival in patients with node-positive TNBC (p=0.018).
Assessing infiltration by CD8-positive TILs in the primary tumor is a useful biomarker to predict pCR and improved outcome in patients with node-positive TNBC.
背景/目的：接受新辅助化疗后获得病理完全缓解（pCR）的三阴性乳腺癌（TNBC）患者预后改善。淋巴细胞为主型乳腺癌更有可能对新辅助化疗有反应。在这里，我们研究了 TNBC 患者新辅助化疗前活检标本中肿瘤浸润淋巴细胞（TIL）与 NAC 反应之间的相关性。
使用多光谱免疫荧光标记检测 52 例 TNBC 患者活检标本中表达免疫细胞谱系表面标志物（CD8、CD4、CD19、CD14、CD11c 和 CD11b）的免疫细胞浸润水平。
pCR 患者的 CD8 阳性 TIL 水平显著更高（p=0.045）。Cox 比例风险模型证实淋巴结受累与无病生存较差相关（p=0.008）。CD8 阳性 TIL 水平高与淋巴结阳性 TNBC 患者无病生存显著延长相关（p=0.018）。
评估原发性肿瘤中 CD8 阳性 TIL 的浸润是预测 pCR 和改善淋巴结阳性 TNBC 患者结局的有用生物标志物。
