Department of Gastrointestinal Surgery, Kanagawa Cancer Center, Yokohama, Japan;
Department of Gastrointestinal Surgery, Kanagawa Cancer Center, Yokohama, Japan.
Anticancer Res. 2024 Oct;44(10):4537-4542. doi: 10.21873/anticanres.17282.
BACKGROUND/AIM: Granzyme B (GZMB) is mainly produced by natural killer (NK) cells and activated CD8-positive T cells to induce tumor cell apoptosis. We analyzed the significance of GZMB expression in gastric cancer (GC) tissues from patients with pathological (p)Stage II/III GC after curative resection.
Patients with pStage II/III GC who received curative resection (n=253) were included and the expression levels of GZMB in GC tissues and in the adjacent normal mucosa were measured using quantitative real-time polymerase chain reaction. The expression levels in GC tissues and clinicopathological features and overall survival (OS) were compared in these patients.
GZMB expression levels were significantly higher in GC tissues than in the adjacent normal mucosa. GZMB expression levels in GC tissues were not associated with any clinicopathological features. The 5-year OS rate in the high-GZMB expression group was significantly better than that in the low-expression group (5-year survival rate 72.0% vs. 55.7%; p=0.009). Furthermore, on multivariate analysis, high-GZMB expression was an independent factor for better OS (hazard ratio=0.652; 95% confidence interval=0.432-0.987; p=0.043).
In patients with locally advanced GC after curative resection, GZMB expression in GC tissue may be a useful prognostic marker.
背景/目的：颗粒酶 B（GZMB）主要由自然杀伤（NK）细胞和活化的 CD8阳性 T 细胞产生，以诱导肿瘤细胞凋亡。我们分析了在接受根治性切除的病理（p）II/III 期胃癌（GC）患者的 GC 组织中 GZMB 表达的意义。
纳入了接受根治性切除的 p 期 II/III 期 GC 患者（n=253），使用实时定量聚合酶链反应测量 GC 组织和相邻正常黏膜中 GZMB 的表达水平。比较了这些患者 GC 组织中的表达水平与临床病理特征和总生存（OS）。
GC 组织中的 GZMB 表达水平明显高于相邻正常黏膜。GC 组织中的 GZMB 表达水平与任何临床病理特征均无关。高 GZMB 表达组的 5 年 OS 率明显优于低表达组（5 年生存率为 72.0% vs. 55.7%；p=0.009）。此外，多因素分析显示，高 GZMB 表达是 OS 更好的独立因素（危险比=0.652；95%置信区间=0.432-0.987；p=0.043）。
在接受根治性切除的局部晚期 GC 患者中，GC 组织中的 GZMB 表达可能是一种有用的预后标志物。
