Dada M O, Campbell G T, Blake C A
Endocrinology. 1983 Sep;113(3):970-84. doi: 10.1210/endo-113-3-970.
We investigated whether 1) the absolute or the relative numbers of LH and FSH cells change during the rat estrous cycle, 2) the percentages of gonadotrophs that contain LH and/or FSH change during the estrous cycle, and 3) gonadotrophs change in size during the rat estrous cycle. Groups of four female rats were decapitated at one of five different times during the estrous cycle. Four male rats were also decapitated. Serum concentrations of LH and FSH were determined by RIA. Paired horizontal flip-flopped or nonflipped paraffin sections were mounted from the dorsal, middle, and ventral portions of each pituitary gland. In each pair of sections, one was stained with a-rat LH-S4 and the other with a-rat FSH-S7 by the unlabeled antibody peroxidase-antiperoxidase method. All immunoreactive cells were counted. Photographs were taken from randomly chosen corresponding areas, and the cells were individually matched to determine the percentage that contained one or both hormones. Correction factors had to be used because in paired flip-flopped or nonflipped sections stained with the same antibody (a-rat LH-S4), not all of the stained cells found in one section were found in the other section. The absolute numbers of LH and FSH cells did not change throughout the estrous cycle. The ratio of LH cells to FSH cells in the pars distalis of female rats was also constant throughout the estrous cycle. In female rats, 75.2% of LH cells also contained FSH, while 99.4% of FSH cells also contained LH. In the male rats, 88.6% of LH cells also contained FSH, while 98.6% of FSH cells also contained LH. Similar results were obtained in paired flip-flopped sections stained with a-rat LH beta and a-rat FSH beta. Sequential staining of additional individual tissue sections with a-rat LH-S4 and then a-rat FHS-S7 or vice versa revealed the following. Staining of LH-stained tissue for FSH revealed less than 1% new cells, but staining of FSH-stained tissue for LH revealed a 8.7% increase in gonadotrophs in males and a 25.4% increase in females. The gonadotrophs in female rats did not change in size during the estrous cycle and were significantly smaller than the gonadotrophs in male rats. The results suggest that in normal adult rats: 1) virtually all FSH-containing cells contain LH, 2) about 25% of the gonadotrophs in females and about 11% of the gonadotrophs in males contain LH but not FSH, 3) the number of cells containing LH or those containing LH and FSH does not change during the estrous cycle, 4) gonadotrophs in female rats do not change in size during the estrous cycle and are smaller than the gonadotrophs in male rats, and 5) FSH release during the early morning of estrus, when the serum FSH concentration is elevated and the serum LH concentration is low, occurs from cells that contain both LH and FSH.
1）大鼠发情周期中促黄体生成素（LH）和促卵泡生成素（FSH）细胞的绝对数量或相对数量是否发生变化；2）含LH和/或FSH的促性腺激素细胞百分比在发情周期中是否改变；3）大鼠发情周期中促性腺激素细胞大小是否改变。在发情周期的五个不同时间点之一，将每组四只雌性大鼠断头处死。还将四只雄性大鼠断头处死。通过放射免疫分析法（RIA）测定血清LH和FSH浓度。从每个垂体的背侧、中间和腹侧部分制作成对的水平翻转或未翻转的石蜡切片。在每对切片中，一张用抗大鼠LH - S4染色，另一张用抗大鼠FSH - S7通过未标记抗体过氧化物酶 - 抗过氧化物酶法染色。对所有免疫反应性细胞进行计数。从随机选择的相应区域拍摄照片，并将细胞逐个匹配以确定含一种或两种激素的细胞百分比。由于在用相同抗体（抗大鼠LH - S4）染色的成对翻转或未翻转切片中，并非在一个切片中发现的所有染色细胞都能在另一个切片中找到，所以必须使用校正因子。LH和FSH细胞的绝对数量在整个发情周期中没有变化。雌性大鼠远侧部中LH细胞与FSH细胞的比例在整个发情周期中也保持恒定。在雌性大鼠中，75.2%的LH细胞也含有FSH，而99.4%的FSH细胞也含有LH。在雄性大鼠中，88.6%的LH细胞也含有FSH，而98.6%的FSH细胞也含有LH。在用抗大鼠LHβ和抗大鼠FSHβ染色的成对翻转切片中获得了类似结果。用抗大鼠LH - S4然后抗大鼠FHS - S7或反之对额外的单个组织切片进行连续染色，结果如下。对LH染色组织进行FSH染色显示新细胞少于1%，但对FSH染色组织进行LH染色显示雄性促性腺激素细胞增加8.7%，雌性增加25.4%。雌性大鼠的促性腺激素细胞在发情周期中大小没有变化，并且明显小于雄性大鼠的促性腺激素细胞。结果表明，在正常成年大鼠中：1）几乎所有含FSH的细胞都含有LH；2）雌性中约25%的促性腺激素细胞和雄性中约11%的促性腺激素细胞含有LH但不含FSH；3）含LH或含LH和FSH的细胞数量在发情周期中不改变；4）雌性大鼠的促性腺激素细胞在发情周期中大小不改变且小于雄性大鼠的促性腺激素细胞；5）在发情期清晨血清FSH浓度升高而血清LH浓度低时，FSH释放来自既含LH又含FSH的细胞。
