The Polymorphism does not Associate with Susceptibility to Typhoid in Punjabi Population, Pakistan: A Case Control Study.

BACKGROUND

SNP based association studies have revolutionized the field of biomedicines. Enteric fever is a systemic disease with etiologic agent serovar typhi and . It is a serious health issue worldwide and presents wide variations in incidence, rates, and severity. Previous investigations revealed that genetic variations may lead to susceptibility to typhoid fever. The current study was performed to investigate the potential association of polymorphism with the susceptibility to typhoid in the Punjabi population.

METHODS

For this case-control study, blood samples obtained from typhoid patients with positive Typhidot or blood culture test (n=72) and healthy controls (n=73) were processed for DNA extraction. The polymorphism analysis was carried out by using PCR and RFLP.

RESULTS

No allelic association was found between and susceptibility to typhoid fever in the understudy population.

CONCLUSION

This case control study is the demonstration of the non-association of with typhoid in the Pakistani population. Future research, using larger population size, will help to elucidate the role of polymorphism in typhoid pathogenesis.