噬菌体MS2的结晶

Crystallization of bacteriophage MS2.

作者信息

Min Jou W, Raeymaekers A, Fiers W

出版信息

Eur J Biochem. 1979 Dec 17;102(2):589-94. doi: 10.1111/j.1432-1033.1979.tb04276.x.

DOI:10.1111/j.1432-1033.1979.tb04276.x

Abstract

Crystals of bacteriophage MS2 have been obtained by slowly cooling a 1% virus solution from 23 degrees C to 0 degrees C in the presence of poly(ethylene glycol) 6000. The crystals were colorless, needle-like, anisotropic and very fragile. Electron microscopic observation of the crystals revealed a two-dimensional lattice of particles with RNA phage morphology and dimensions. Preliminary X-ray examination of the crystals confirmed their viral nature.

摘要

通过在聚乙二醇6000存在的情况下，将1%的病毒溶液从23摄氏度缓慢冷却至0摄氏度，获得了噬菌体MS2的晶体。这些晶体无色、呈针状、各向异性且非常易碎。对晶体的电子显微镜观察显示，其粒子呈二维晶格排列，具有RNA噬菌体的形态和尺寸。对晶体的初步X射线检查证实了它们的病毒性质。