噬菌体fr及其重组衣壳的结晶
Crystallization of bacteriophage fr and its recombinant capsids.
作者信息
Bundule M, Pumpens P, Ose V, Valegård K, Liljas L
机构信息
Institute of Molecular Biology, Latvian Academy of Sciences, Riga.
出版信息
J Mol Biol. 1993 Aug 5;232(3):1005-6. doi: 10.1006/jmbi.1993.1451.
PMID:8355265
Abstract
Single crystals of Escherichia coli bacteriophage fr and its recombinant capsids have been obtained by the vapour diffusion technique in the presence of ammonium sulphate. They diffract X-rays to at least 3.5 A. Electron microscopic observation of the crystals revealed a three-dimensional lattice of particles with RNA phage morphology and dimensions.
摘要
通过在硫酸铵存在下的气相扩散技术，已获得大肠杆菌噬菌体fr及其重组衣壳的单晶。它们对X射线的衍射至少可达3.5埃。对晶体的电子显微镜观察显示出具有RNA噬菌体形态和尺寸的颗粒的三维晶格。
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