Mwamula Abraham Okki, Lee Sang Myeong, Jung Young Hak, Kim Yi Seul, Lee Dong Woon
Research Institute of Invertebrate Vector, Kyungpook National University, Sangju 37224, Republic of Korea.
SM Biovision Co., Jinju, 52849, Republic of Korea.
J Nematol. 2024 Sep 30;56(1):20240028. doi: 10.2478/jofnem-2024-0028. eCollection 2024 Mar.
n. sp., a new species isolated from the bark and cambium layer of a dead black pine tree is characterized herein using integrative taxonomy, considering both morphological and molecular phylogenetic analyses of the 18S- and 28S-rRNA genes. n. sp. is characterized by having a medium-sized body 1.50-1.89 mm long; lip region angular and offset by a depression; a relatively long odontostyle (17.0-19.0 μm); vulval opening a transverse slit, positioned slightly posteriorly; with two elongated drop-shaped to spindle-shaped sclerotizations; an intestine-prerectum junction with a long anteriorly directed conical or tongue-like projection; a relatively long female tail (115-187 μm); spicules 48.0-57.0 μm long; and regularly spaced 7-8 ventromedian supplements. It is closest to , especially in having similar body length and number of ventromedian supplements but can be differentiated from by the longer odontostyle, tongue-like projection, and longer spicules. The phylogenies based on the 18S- and 28S-rRNA sequences showed a well-supported sister relation of n. sp. with , and other member species of the group.
新种，一种从枯死黑松树的树皮和形成层分离出的新物种，本文采用综合分类法进行特征描述，同时考虑了18S和28S核糖体RNA基因的形态学和分子系统发育分析。新种的特征为：身体中等大小，长1.50 - 1.89毫米；唇区呈角状，由一个凹陷隔开；齿针相对较长（17.0 - 19.0微米）；阴门开口为横向裂缝，位置略靠后；有两个细长的滴状至纺锤形硬化；肠 - 直肠交界处有一个向前的长锥形或舌状突起；雌性尾部相对较长（115 - 187微米）；交合刺长48.0 - 57.0微米；腹中线有7 - 8个规则间隔的副器。它与[某物种]最为接近，特别是在体长和腹中线副器数量方面相似，但可以通过较长的齿针、舌状突起和较长的交合刺与[某物种]区分开来。基于18S和28S核糖体RNA序列的系统发育显示，新种与[某物种]以及该类群的其他成员物种形成了一个得到充分支持的姐妹关系。
