Department of Zoology, Shaheed Benazir Bhutto Women University, Charsadda Road, Peshawar, Pakistan.
Virology Section, Center of Microbiology and Biotechnology, Veterinary Research Institute Bacha Khan Chowk, Peshawar, Pakistan.
Mol Biol Rep. 2024 Oct 1;51(1):1032. doi: 10.1007/s11033-024-09910-x.
Peste des Petits Ruminants (PPR) is an economically significant transboundary viral disease of sheep and goats caused by the PPRV virus, affecting annual losses of 1.45-2.10 billion US dollars globally. We designed the current study to evaluate the positive cases, molecular characterization, phylogenetic analysis, and risk factors correlated with the disease in various districts of Khyber Pakhtunkhwa, Pakistan, with the aim of contributing to these strategies.
A total of 384 samples from three selected districts, i.e., Peshawar, Charsadda and Chitral (n = 128 each), were collected, and the virus was investigated by using the sandwich ELISA, while the N gene of the virus was used as a target for molecular detection via RT-PCR. The confirmed samples were then sequenced, and phylogenetic analysis was performed. According to our findings, the highest positive cases was found in district Peshawar (50.87%), followed by Charsadda and Chitral (24.56%), respectively, while risk factor analysis showed that certain categories, such as species, sex, and age less than two years, have higher risk (P < 0.05) in contrast to their respective categories. Furthermore, sequencing and phylogenetic analysis of representative samples showed that the PPRV strains in the current study clustered in lineage IV, which is circulating in the small ruminant population of Asia, the Middle East, and African countries. Comparative residue analysis highlighted the mutation by representing 242 variable sites out of 371 locations.
PPRV has foremost importance in Pakistan because the virus was detected in a considerable number of samples, and most of which were sourced from subsidiary areas where veterinary services are not prioritized.
小反刍兽疫（PPR）是一种由小反刍兽疫病毒（PPRV）引起的具有重要经济意义的绵羊和山羊的跨境病毒性疾病，对全球造成的年度损失为 14.5 亿至 21 亿美元。我们设计了本研究，以评估在巴基斯坦开伯尔-普赫图赫瓦省的不同地区与该疾病相关的阳性病例、分子特征、系统进化分析和风险因素，旨在为这些策略做出贡献。
从三个选定的地区（白沙瓦、查沙达和奇特拉尔）共采集了 384 份样本（每个地区 128 份），使用夹心 ELISA 检测病毒，并用 RT-PCR 检测病毒的 N 基因作为分子检测的靶标。然后对确认的样本进行测序和系统进化分析。根据我们的研究结果，白沙瓦区的阳性病例最高（50.87%），其次是查沙达和奇特拉尔（分别为 24.56%），而风险因素分析表明，某些类别，如物种、性别和年龄小于两岁，与各自的类别相比，风险更高（P<0.05）。此外，对代表性样本的测序和系统进化分析表明，本研究中的 PPRV 株在亚洲、中东和非洲国家的小反刍动物群体中流行的第四谱系中聚集。比较残基分析通过代表 371 个位置中的 242 个可变位点突出了突变。
PPRV 在巴基斯坦具有首要重要性，因为在相当数量的样本中检测到了病毒，而且大多数样本都来自兽医服务不是优先考虑的附属地区。
