Department of Food Science, National Taiwan Ocean University, No. 2, Beining Road, Keelung 20224, Taiwan, ROC; Testing Laboratory, Creation Food Co. Ltd., 3F No. 9, Ln. 168, Xingshan Road, Taipei 114066, Taiwan, ROC.
Department of Food Science, National Taiwan Ocean University, No. 2, Beining Road, Keelung 20224, Taiwan, ROC.
Food Chem. 2025 Jan 15;463(Pt 4):141438. doi: 10.1016/j.foodchem.2024.141438. Epub 2024 Sep 25.
Conditions were determined for rapid, convenient, and efficient determination of 16 common mycotoxins in tea samples. Mycotoxins in tea leaves and tea infusion samples were extracted using solid-liquid extraction/liquid-liquid extraction combined with ultrasonic-assisted extraction. The extraction solvent was 2-butanone/ethyl acetate (9/1 v/v) with 0.1 % formic acid. The established conditions enabled the analysis of these mycotoxins by ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) in 5.5 min. In addition, HPLC with a temperature-controlled fluorescence detector was able to simultaneously determine 8 mycotoxins with fluorescent properties in 10 min without derivatization. Aflatoxin M1 (2.15 and 3.01 μg/kg), fumonisin B2 (198.89 μg/kg), and zearalenone (87.54 μg/kg) could be detected in commercially available pu-erh tea, green tea, and black tea products, and their total transfer rates from the products to brewed tea infusions were 64.08-65.13 %, 90.59 %, and 25.99 %, respectively. The risks of drinking mycotoxins from these tea infusions mostly showed low levels of concern.
建立了同时测定茶叶及茶浸提液中 16 种常见真菌毒素的超高效液相色谱-串联质谱(UHPLC-MS/MS)方法和高效液相色谱-荧光检测法(HPLC-FLD)。样品经乙腈提取，采用液液萃取/超声辅助提取(SPE/USA)结合的前处理方法净化，以 2-丁酮/乙酸乙酯(9/1，v/v)为提取溶剂，0.1%甲酸为酸化剂。UHPLC-MS/MS 分析在 5.5 min 内完成，HPLC-FLD 方法在 10 min 内可同时测定 8 种具有荧光特性的真菌毒素，无需衍生化。市售普洱茶、绿茶和红茶产品中均检测到黄曲霉毒素 M1(2.15 和 3.01μg/kg)、伏马菌素 B2(198.89μg/kg)和玉米赤霉烯酮(87.54μg/kg)，其从产品向冲泡茶浸提液中的总转移率分别为 64.08%-65.13%、90.59%和 25.99%。从这些茶浸提液中摄入真菌毒素的风险主要处于低关注水平。
