H10N3禽流感病毒血凝素关键抗原位点的鉴定
Identification of key antigenic sites in hemagglutinin of H10N3 avian influenza virus.
作者信息
Wan Zhimin, Tang Ting, Jiang Wenjie, Zhao Zhehong, Li Yafeng, Xie Quan, Li Tuofan, Shao Hongxia, Qin Aijian, Ye Jianqiang
机构信息
Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 225009, China; Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, College of Veterinary Medicine, Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 225009, China; Institute of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
出版信息
Poult Sci. 2024 Dec;103(12):104343. doi: 10.1016/j.psj.2024.104343. Epub 2024 Sep 19.
The H10 avian influenza viruses (AIV) have been detected in both birds and mammals. Recently, the cases of human infection with H10N8 and H10N3 in China pose high risk to public health. However, the antigenic sites in hemagglutinin (HA) of H10 are poorly understood. In this study, 3 monoclonal antibodies (MAb), designated as 1F4, 6B3 and 6G12, against the HA protein of the H10N3 strain A/chicken/Taizhou/498/2021(H10N3) (TZ498), were first generated. All of these MAb could effectively inhibit TZ498 in haemagglutination inhibition assay and microneutralization assay. Four novel antigenic sites at positions 135, 208, 227, and 266 (H10 numbering) were identified in the HA of TZ498 through escape mutants selected by these 3 MAb. Moreover, natural mutations at positions 135 and 227 were found in the H10 field strains. All these not only provide novel insights into the molecular markers for monitoring the antigenic variation of H10 but also be helpful for developing efficient control strategies against H10.
H10禽流感病毒（AIV）已在鸟类和哺乳动物中被检测到。最近，中国出现的人感染H10N8和H10N3病例对公共卫生构成了高风险。然而，人们对H10血凝素（HA）中的抗原位点了解甚少。在本研究中，首次制备了3种针对H10N3毒株A/鸡/泰州/498/2021（H10N3）（TZ498）HA蛋白的单克隆抗体（MAb），命名为1F4、6B3和6G12。所有这些单克隆抗体在血凝抑制试验和微量中和试验中均能有效抑制TZ498。通过这3种单克隆抗体筛选出的逃逸突变体，在TZ498的HA中鉴定出了位于135、208、227和266位（H10编号）的4个新抗原位点。此外，在H10田间毒株中发现了135和227位的自然突变。所有这些不仅为监测H10抗原变异的分子标志物提供了新的见解，也有助于制定针对H10的有效防控策略。
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