School and Hospital of Stomatology, Tianjin Medical University, 12 Observatory Road, Tianjin, 300070, China.
Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, Tianjin, 300070, China.
Sci Rep. 2024 Oct 1;14(1):22855. doi: 10.1038/s41598-024-69933-z.
Periodontitis is a chronic inflammatory disease involving plaque biofilm as a pathogenic factor. Potassium ion plays an important role in cellular homeostasis; a large outflow of potassium may lead to local inflammation progression. In this work, the multifunctional short peptide molecule BmKTX-33 was designed by modifying the BmKTX, a Kv1.3 potassium channel inhibitor. This was to explore its antibacterial properties, capability of maintaining cell ion homeostasis, and bone-forming capacity. The results showed that BmKTX-33 had inhibitory effects on S. gordonii, F. nucleatum, and P. gingivalis. Moreover, BmKTX-33 also inhibited excessive potassium outflow in inflammatory environments. Finally, BmKTX-33 promoted MC3T3-E1 early osteogenesis while suppressing the NLRP3 inflammasome's production. In conclusion, BmKTX-33 not only has antibacterial properties, but also can inhibit the expression of NLRP3 inflammasome and play an anti-inflammatory role.
牙周炎是一种慢性炎症性疾病，涉及菌斑生物膜作为致病因素。钾离子在细胞内稳态中起着重要作用；钾的大量外流可能导致局部炎症的进展。在这项工作中，通过修饰 Kv1.3 钾通道抑制剂 BmKTX，设计了多功能短肽分子 BmKTX-33。目的是探索其抗菌特性、维持细胞离子稳态的能力和成骨能力。结果表明，BmKTX-33 对 S. gordonii、F. nucleatum 和 P. gingivalis 具有抑制作用。此外，BmKTX-33 还抑制了炎症环境中钾的过度外流。最后，BmKTX-33 促进 MC3T3-E1 早期成骨，同时抑制 NLRP3 炎性小体的产生。总之，BmKTX-33 不仅具有抗菌特性，还能抑制 NLRP3 炎性小体的表达，发挥抗炎作用。
