School of Biological Sciences, The University of Hong Kong, Hong Kong, SAR, China.
Methods Mol Biol. 2025;2855:171-183. doi: 10.1007/978-1-0716-4116-3_11.
Oxidative stress induces autooxidation of polyunsaturated fatty acids, producing numerous isoprostanoids and isofuranoids. These oxidized products are measurable in human plasma and urine and serve as oxidative stress biomarkers for chronic diseases. This chapter details the preparation and measurement of α-linolenic acid-derived phytoprostanes and phytofurans in human samples using liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-QToF-MS/MS).
氧化应激会导致多不饱和脂肪酸的自动氧化，产生大量的异前列腺素和异呋喃素。这些氧化产物可在人血浆和尿液中测量，并作为慢性疾病的氧化应激生物标志物。本章详细介绍了使用液相色谱-四极杆飞行时间串联质谱（LC-QToF-MS/MS）在人样本中制备和测量α-亚麻酸衍生的植物前列腺素和植物呋喃素的方法。
