Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Madrid, Spain.
Methods Mol Biol. 2025;2855:389-423. doi: 10.1007/978-1-0716-4116-3_23.
Capillary electrophoresis coupled to mass spectrometry (CE-MS) has emerged as a powerful analytical technique with significant implications for clinical research and diagnostics. The integration of information from CE and MS strengthens confidence in the identification of compounds present in clinical samples. The ability of CE to separate molecules based on their electrophoretic mobility coupled to MS enables the accurate identification and quantification of analytes, even in complex biological matrices such as human plasma.Here, we present a detailed protocol for an untargeted metabolomics study using CE-MS and its application in a study on human plasma from patients suffering Long COVID syndrome. The protocol ranges from sample preparation to biological interpretation, detailing a workflow enabling the analysis of cationic and anionic compounds, metabolite identification, and data processing.
毛细管电泳与质谱联用（CE-MS）已成为一种强大的分析技术，对临床研究和诊断具有重要意义。CE 和 MS 信息的整合增强了对临床样本中存在化合物的鉴定信心。CE 根据其电泳迁移率分离分子的能力与 MS 相结合，使分析物的准确鉴定和定量成为可能，即使在复杂的生物基质如人血浆中也是如此。在这里，我们介绍了一种使用 CE-MS 进行非靶向代谢组学研究的详细方案及其在长新冠综合征患者血浆研究中的应用。该方案从样品制备到生物解释，详细说明了一个工作流程，能够分析阳离子和阴离子化合物、代谢物鉴定和数据处理。
