Matsubara Joji, Kumagai Ken, Ishikawa Kimi, Choe Hyonmin, Ike Hiroyuki, Kobayashi Naomi, Inaba Yutaka
Department of Orthopaedic Surgery, Graduate School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan.
Department of Orthopaedic Surgery, Yokohama City University Medical Center, Yokohama, Japan.
BMC Musculoskelet Disord. 2024 Oct 1;25(1):759. doi: 10.1186/s12891-024-07886-0.
This study aimed to investigate the expression of vascular endothelial growth factor (VEGF) in cruciate ligaments from patients with osteoarthritis (OA). It was hypothesized that the expression level of VEGF is associated with the extent of degeneration of the cruciate ligaments.
Remnants of anterior cruciate ligaments (ACLs) from patients with acute ACL injury due to trauma, and ACLs and posterior cruciate ligaments (PCLs) from patients with primary OA were assessed histologically. Samples were immunohistochemically stained with VEGF and tenomodulin, and immunopositive cells were quantitatively assessed by the histological grades of ligament degeneration.
Histological analysis showed significant degeneration of the ACLs from OA patients compared with trauma patients, with increased expression of VEGF correlating with higher grades of degeneration. Conversely, tenomodulin expression was lower in more degenerated cruciate ligaments. The percentage of VEGF-positive cells was correlated inversely with that of tenomodulin-positive cells.
Increased VEGF expression is associated with degeneration of cruciate ligaments in patients with osteoarthritis of the knee.
本研究旨在调查骨关节炎（OA）患者十字韧带中血管内皮生长因子（VEGF）的表达情况。研究假设VEGF的表达水平与十字韧带的退变程度相关。
对因创伤导致急性前交叉韧带（ACL）损伤患者的ACL残端，以及原发性OA患者的ACL和后交叉韧带（PCL）进行组织学评估。样本用VEGF和腱调蛋白进行免疫组织化学染色，免疫阳性细胞通过韧带退变的组织学分级进行定量评估。
组织学分析显示，与创伤患者相比，OA患者的ACL有明显退变，VEGF表达增加与更高程度的退变相关。相反，在退变更严重的十字韧带中，腱调蛋白表达较低。VEGF阳性细胞百分比与腱调蛋白阳性细胞百分比呈负相关。
VEGF表达增加与膝关节骨关节炎患者十字韧带退变相关。
