Andrews N W, Alves M J, Schumacher R I, Colli W
Exp Parasitol. 1985 Dec;60(3):255-62. doi: 10.1016/0014-4894(85)90029-3.
Trypomastigote forms from the Y strain of Trypanosoma cruzi were inactivated by treatment with 8-methoxypsoralen and ultraviolet radiation (365 nm). The parasite population maintained normal morphology, mobility, and mammalian cell invasion capacity, being incapable of intracellular differentiation and reproduction. A strong protection of inbred A/Snell mice against challenges with virulent T. cruzi forms was obtained through three inoculations of the inactivated trypomastigotes. All immunized mice survived, with negative parasitemias and absence of tissue lesions. Several antibody-mediated reactions were performed with sera from the protected mice at distinct stages of the experiment. The levels of agglutinating, lytic (complement-mediated), and protein A binding antibodies increased progressively with each immunizing booster. The trypomastigote surface proteins recognized by antibodies present in these sera were identified after immunoprecipitation and two-dimensional polyacrylamide gel electrophoresis.
用8-甲氧基补骨脂素和紫外线（365nm）处理克氏锥虫Y株的锥鞭毛体形式可使其失活。寄生虫群体保持正常形态、运动能力和哺乳动物细胞侵袭能力，但无法进行细胞内分化和繁殖。通过三次接种灭活的锥鞭毛体，获得了对强毒克氏锥虫形式攻击的近交A/Snell小鼠的强大保护。所有免疫小鼠均存活，无寄生虫血症且无组织损伤。在实验的不同阶段，用受保护小鼠的血清进行了几种抗体介导的反应。随着每次免疫加强，凝集、溶解（补体介导）和蛋白A结合抗体的水平逐渐增加。经免疫沉淀和二维聚丙烯酰胺凝胶电泳后，鉴定了这些血清中存在的抗体所识别的锥鞭毛体表面蛋白。
