Yoshida N
Exp Parasitol. 1986 Apr;61(2):184-91. doi: 10.1016/0014-4894(86)90151-7.
The complement-dependent lytic activity of various mouse antisera to Trypanosoma cruzi was tested, using purified culture metacyclic trypomastigotes as targets, in an attempt to verify the correlation between resistance to T. cruzi and lytic antibodies, Sera of mice immunized with killed trypomastigotes, which resisted acute infection by insect-derived metacyclics of T. cruzi, consistently lysed over 95% of trypomastigotes in vitro. In contrast, fewer than 50% of parasites were lysed by sera of mice immunized with killed epimastigotes, which, upon challenge with metacyclic forms, developed high parasitemias comparable to those of non-immunized controls. All immune sera with high lytic activity, namely sera of mice immunized with killed trypomastigotes and sera of chronically infected mice, reacted with a set of 131I-labeled trypomastigote surface proteins with apparent molecular weights of 77,000, 82,000, and 88,000, which failed to be recognized by sera of mice immunized with epimastigotes, Absorption of lytic sera with trypomastigotes, but not with epimastigotes, greatly reduced the ability to lyse trypomastigotes and to precipitate the surface polypeptides.
以纯化的培养循环后期锥鞭毛体为靶标，检测了各种小鼠抗克氏锥虫血清的补体依赖性溶解活性，以试图验证对克氏锥虫的抗性与溶解抗体之间的相关性。用杀死的锥鞭毛体免疫的小鼠血清能够抵抗克氏锥虫昆虫源性循环后期锥鞭毛体的急性感染，在体外能持续溶解超过95%的锥鞭毛体。相比之下，用杀死的上鞭毛体免疫的小鼠血清只能溶解不到50%的寄生虫，这些小鼠在用循环后期锥鞭毛体攻击后，会出现与未免疫对照组相当的高寄生虫血症。所有具有高溶解活性的免疫血清，即经杀死的锥鞭毛体免疫的小鼠血清和慢性感染小鼠的血清，都能与一组131I标记的锥鞭毛体表面蛋白发生反应，这些蛋白的表观分子量分别为77000、82000和88000，而经上鞭毛体免疫的小鼠血清无法识别这些蛋白。用锥鞭毛体而非上鞭毛体吸收溶解血清，可大大降低溶解锥鞭毛体和沉淀表面多肽的能力。
