Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.
Avtsyn Research Institute of Human Morphology of FSBSI "Petrovsky National Research Centre of Surgery", Moscow, Russia.
J Biophotonics. 2024 Nov;17(11):e202400368. doi: 10.1002/jbio.202400368. Epub 2024 Oct 2.
One of the urgent tasks of modern medicine is to detect microcirculation disorder during surgery to avoid possible consequences like tissue hypoxia, ischemia, and necrosis. To address this issue, in this article, we propose a compact probe with sapphire tip and optical sensing based on the principle of spatially resolved diffuse reflectance analysis. It allows for intraoperative measurement of tissue effective attenuation coefficient and its alteration during the changes of tissue condition, caused by microcirculation disorder. The results of experimental studies using (1) a tissue-mimicking phantom based on lipid emulsion and hemoglobin and (2) a model of hindlimb ischemia performed in a rat demonstrated the ability to detect rapid changes of tissue attenuation confirming the feasibility of the probe to sense the stressful exposure. Due to a compact design of the probe, it could be useful for rather wide surgical operations and diagnostic purposes as an auxiliary instrument.
现代医学的当务之急之一是在手术过程中检测微循环障碍，以避免组织缺氧、缺血和坏死等可能的后果。针对这一问题，本文提出了一种基于空间分辨漫反射分析原理的带有蓝宝石尖端和光学传感的紧凑型探头，可实现在手术过程中测量组织有效衰减系数及其在组织条件变化过程中的变化，而组织条件的变化是由微循环障碍引起的。使用（1）基于脂乳和血红蛋白的组织模拟体和（2）在大鼠中进行的后肢缺血模型进行的实验研究结果表明，该探头能够检测到组织衰减的快速变化，证实了该探头探测应激暴露的可行性。由于探头的紧凑设计，它可作为一种辅助仪器，在相当广泛的手术和诊断中具有应用价值。
