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该基因在烟草中的过表达增强了萜类化合物的合成。

Overexpression of the gene in tobacco enhances terpenoid compounds synthesis.

作者信息

Wu Junpeng, Bu Manli, Zong Yaxian, Tu Zhonghua, Cheng Yanli, Li Huogen

机构信息

Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China.

College of architecture, Anhui Science and Technology University, Bengbu, Anhui, China.

出版信息

Front Plant Sci. 2024 Sep 17;15:1445103. doi: 10.3389/fpls.2024.1445103. eCollection 2024.

DOI:10.3389/fpls.2024.1445103
PMID:39354939
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11442295/
Abstract

, a relic genus from the Magnoliaceae family, comprises two species, and . is distinguished by its extensive natural distribution in Eastern North America. Conversely, is nearing endangerment due to its low regeneration rate. A pivotal aspect in the difference of these species involves terpenoids, which play crucial roles in plant growth and attracting pollinators. However, the complex molecular mechanisms underlying terpenoid roles in are not well understood. Terpene Synthases (TPS) genes are widely reported to play a role in terpenoid biosynthesis, hence, this study centers on genes in spp. Employing multiple bioinformatics methods, a differential expression gene in , , was discerned for further functional analysis. Subcellular localization results reveal the involvement of in chloroplast-associated processes, hence participate in terpenoid biosynthesis within chloroplasts. Heterologous transformation of the gene into tobacco significantly elevates the levels of common terpenoid compounds, including chlorophyll, gibberellin, and carotenoids. Collectively, these findings not only underscore the role of the gene in the biosynthesis of terpenoids but also lay a foundation for future research on interspecific differences in .

摘要

木兰科的一个残遗属包含两个物种，[物种名1]和[物种名2]。[物种名1]的特点是在北美东部有广泛的自然分布。相反，[物种名2]由于其低再生率而濒临灭绝。这些物种差异的一个关键方面涉及萜类化合物，它们在植物生长和吸引传粉者方面发挥着关键作用。然而，萜类化合物在[物种名2]中发挥作用的复杂分子机制尚不清楚。萜烯合酶（TPS）基因被广泛报道在萜类生物合成中起作用，因此，本研究聚焦于[物种名2]中的TPS基因。采用多种生物信息学方法，在[物种名2]中识别出一个差异表达基因进行进一步的功能分析。亚细胞定位结果表明[基因名]参与叶绿体相关过程，因此参与叶绿体中的萜类生物合成。将[基因名]基因异源转化到烟草中显著提高了包括叶绿素、赤霉素和类胡萝卜素在内的常见萜类化合物的水平。总的来说，这些发现不仅强调了[基因名]基因在萜类生物合成中的作用，也为未来关于[物种名2]种间差异的研究奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/3e0db09fc652/fpls-15-1445103-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/f2d0a68c85ab/fpls-15-1445103-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/cb45f9b83bee/fpls-15-1445103-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/d390a6dda196/fpls-15-1445103-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/f3cd41464d1d/fpls-15-1445103-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/3e0db09fc652/fpls-15-1445103-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/f7c7a789eab8/fpls-15-1445103-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/ac9d18120d44/fpls-15-1445103-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/5b4976406701/fpls-15-1445103-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/9f3db0f2c834/fpls-15-1445103-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/1eaf60b1b88a/fpls-15-1445103-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/cb45f9b83bee/fpls-15-1445103-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/d390a6dda196/fpls-15-1445103-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/f3cd41464d1d/fpls-15-1445103-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e33/11442295/3e0db09fc652/fpls-15-1445103-g010.jpg

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