Tonsil explants as a human model to study vaccine responses.

INTRODUCTION

Vaccination is one of the most effective infection prevention strategies. Viruses with high mutation rates -such as influenza- escape vaccine-induced immunity and represent significant challenges to vaccine design. Influenza vaccine strain selection is based on circulating strains and immunogenicity testing in animal models with limited predictive outcomes for vaccine effectiveness in humans.

METHODS

We developed a human vaccination model using human tonsil tissue explants cultured in 3D perfusion bioreactors to be utilized as a platform to test and improve vaccines.

RESULTS

Tonsils cultured in bioreactors showed higher viability, metabolic activity, and more robust immune responses than those in static cultures. The vaccination system responded to various premanufactured vaccines, protein antigens, and antigen combinations. In particular, a multivalent immunization with three phylogenetically distant H3N2 influenza strains showed evidence for broader B cell activation and induced higher antibody cross-reactivity than combinations with more related strains. Moreover, we demonstrate the capacity of our model to generate de novo humoral immune responses to a model antigen.

DISCUSSION

Perfusion-cultured tonsil tissue may be a valuable human model for immunology research with potential application in vaccine candidate selection.