Mohammad Ali Shariatzadeh Seyed, Salmani Fatemeh, Moghanlo Hossein, Mahmoodi Monireh
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Int J Reprod Biomed. 2024 Sep 12;22(7):553-566. doi: 10.18502/ijrm.v22i7.16964. eCollection 2024 Jul.
The toxicity of silver nanoparticles (AgNPs) has been proven in the female reproductive system. Thymoquinone (TQ) is a natural antioxidant and bioactive component of .
We evaluated the efficacy of TQ on ovarian tissue following toxicity induced by AgNPs in female mice.
24 female NMRI mice (5-6 wk, an average weight of 33 gr) were randomly divided into 4 groups (n = 6/each): control, AgNPs (500 mg/kg, gavage), TQ (2.5 mg/kg, intraperitoneal injection), and TQ+AgNPs. Mice were treated every day for 35 days. Serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), luteinizing hormone, and follicle-stimulating hormone were measured. The optical disector and stereological techniques were utilized to estimate the follicular count, their volume at different developmental stages, and the structure of ovarian tissue.
In the AgNPs group, the serum concentrations of TAC (p = 0.01), luteinizing hormone (p 0.001), follicle-stimulating hormone, the volume of corpus luteum (p 0.001), and the number of follicles decreased significantly compared to the control group. Nevertheless, AgNPs significantly increased the MDA level. In the TQ+AgNPs group compared to the AgNPs group, a significant decrease in MDA level (p 0.001) and a significant improvement in TAC (p = 0.03), and hormonal levels, the number of primary, preantral, and antral follicles (p = 0.04), and the volume of corpus luteum (p = 0.01) were observed.
TQ improved the number of follicles by reducing oxidative stress and lipid peroxidation in AgNPs-damaged ovarian tissue.
银纳米颗粒（AgNPs）的毒性已在雌性生殖系统中得到证实。百里醌（TQ）是一种天然抗氧化剂和[某种物质]的生物活性成分。
我们评估了TQ对雌性小鼠中由AgNPs诱导毒性后的卵巢组织的功效。
将24只雌性NMRI小鼠（5 - 6周龄，平均体重33克）随机分为4组（每组n = 6）：对照组、AgNPs组（500毫克/千克，灌胃）、TQ组（2.5毫克/千克，腹腔注射）和TQ + AgNPs组。小鼠每天接受治疗，持续35天。测量血清丙二醛（MDA）水平、总抗氧化能力（TAC）、促黄体生成素和促卵泡激素水平。利用光学分割器和体视学技术估计卵泡数量、不同发育阶段卵泡的体积以及卵巢组织的结构。
与对照组相比，AgNPs组血清TAC浓度（p = 0.01）、促黄体生成素（p < 0.001）、促卵泡激素、黄体体积（p < 0.001）和卵泡数量显著降低。然而，AgNPs显著提高了MDA水平。与AgNPs组相比，TQ + AgNPs组MDA水平显著降低（p < 0.001），TAC（p = 0.03）、激素水平、初级卵泡、窦前卵泡和窦状卵泡数量（p = 0.04）以及黄体体积（p = 0.01）显著改善。
TQ通过减少AgNPs损伤的卵巢组织中的氧化应激和脂质过氧化作用，提高了卵泡数量。
