Tchurikov N A, Vartanian A A, Klushevskaya E S, Alembekov I R, Kretova A N, Chechetkin V R, Kravatskaya G I, Kosorukov V S, Kravatsky Y V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Department of Experimental Diagnosis and Therapy of Tumors, N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of Russia, Moscow, 115478 Russia.
Mol Biol (Mosk). 2024 Mar-Apr;58(2):295-304.
Multiple exogenous or endogenous factors alter gene expression patterns by different mechanisms that are poorly understood. We used RNA-Seq analysis in order to study changes in gene expression in melanoma cells that are capable of vasculogenic mimicry that is inhibited upon the action of an inhibitor of vasculogenic mimicry. Here, we show that the drug induces a strong upregulation of 50 genes that control the cell cycle and microtubule cytoskeleton coupled with a strong downregulation of 50 genes that control different cellular metabolic processes. We found that both groups of genes are simultaneously regulated by multiple sets of transcription factors. We conclude that one way for coordinated regulation of large groups of genes is regulation simultaneously by multiple transcription factors.
多种外源性或内源性因素通过尚不清楚的不同机制改变基因表达模式。我们使用RNA测序分析来研究黑色素瘤细胞中基因表达的变化，这些黑色素瘤细胞能够形成血管生成拟态，而血管生成拟态在血管生成拟态抑制剂的作用下会受到抑制。在此，我们表明该药物诱导了50个控制细胞周期和微管细胞骨架的基因强烈上调，同时50个控制不同细胞代谢过程的基因强烈下调。我们发现这两组基因同时受到多组转录因子的调控。我们得出结论，对大量基因进行协调调控的一种方式是由多个转录因子同时进行调控。
