通过基因组组装和全基因组关联分析鉴定黄皮果实中控制糖和有机酸积累的关键基因

Identification of Key Genes Controlling Sugar and Organic Acid Accumulation in Wampee Fruit () via Genome Assembly and Genome-wide Association Analysis.

作者信息

Chen Huiqiong, Wang Jingxuan, Wang Xiangfeng, Peng Cheng, Chang Xiaoxiao, Chen Zhe, Yang Bowen, Wang Xinrui, Qiu Jishui, Guo Li, Lu Yusheng

机构信息

Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Science and Technology Research on Fruit Trees, Guangzhou 510640, China.

Shandong Provincial Key Laboratory of Precision Molecular Crop Design and Breeding, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Weifang, Shandong 261325, China.

出版信息

J Agric Food Chem. 2024 Oct 2. doi: 10.1021/acs.jafc.4c04830.

DOI:10.1021/acs.jafc.4c04830

PMID:39356738

Abstract

Wampee () is an economically significant subtropical fruit tree widely cultivated in Southern China. To provide high-quality genomic resources for , we report a chromosome-level genome sequence for the "JinFeng" cultivar. The 297.1 Mb genome contained nine chromosomes with a scaffold N50 of 29.2 Mb and encoded 23,468 protein-coding genes. Selective sweep analysis between sweet and sour varieties and genome-wide association analysis identified 14 candidate genes putatively involved in sugar and acid accumulation. , encoding an ethylene response factor, and , encoding a Sugars Will Eventually be Exported Transporters (SWEET) family protein, were proposed as key regulators of the sweet and sour tastes of the wampee fruit. and overexpression in tomatoes increased the total sugar and acid content in fruits. promoter activation by ClERF061 was confirmed via transient expression. Our study provides valuable genomic resources for genetics and breeding.

摘要

黄皮（）是一种具有重要经济价值的亚热带果树，在中国南方广泛种植。为了为其提供高质量的基因组资源，我们报道了“金凤”品种的染色体水平基因组序列。297.1 Mb的基因组包含9条染色体，支架N50为29.2 Mb，编码23,468个蛋白质编码基因。甜黄皮和酸黄皮品种之间的选择性清除分析以及全基因组关联分析确定了14个可能参与糖和酸积累的候选基因。编码乙烯反应因子的和编码糖最终输出转运蛋白（SWEET）家族蛋白的被认为是黄皮果实酸甜味道的关键调节因子。在番茄中过表达和增加了果实中的总糖和酸含量。通过瞬时表达证实了ClERF061对启动子的激活作用。我们的研究为黄皮的遗传和育种提供了有价值的基因组资源。