Chew B P, Tjoelker L W, Tanaka T S
J Dairy Sci. 1985 Nov;68(11):3037-46. doi: 10.3168/jds.S0022-0302(85)81199-1.
Antimicrobial activities of three phenolic compounds and four metal chelators were tested at 0, 250, 500, and 1000 ppm in vitro against four major mastitis-causing bacteria, Streptococcus agalactiae, Staphylococcus aureus, Klebsiella pneumoniae, and Escherichia coli. Overall, butylated hydroxyanisole and tert-butylhydroquinone showed the greatest antimicrobial activity. These phenolics were bactericidal at 250 to 500 ppm against all four bacteria tested. The butylated hydroxytoluene was bactericidal against the gram-positive bacteria but was ineffective against the coliforms. At 250 ppm, disodium ethylenediaminetetraacetic acid was bactericidal against the gram-positive bacteria but much less effective against the gram-negatives. However, diethylenetriaminepentaacetic acid was more growth inhibitory than ethylenediaminetetraacetic acid against the gram-negative bacteria and especially against Escherichia coli. All other compounds were generally much less effective or ineffective against all four microorganisms. Therefore, butylated hydroxyanisole, butylated hydroxytoluene, tert-butylhydroquinone, ethylenediaminetetraacetic acid, and diethylenetriaminepentaacetic acid may have practical implications in the prevention or treatment of bovine mastitis.
在体外，对三种酚类化合物和四种金属螯合剂在0、250、500和1000 ppm浓度下针对四种主要引起乳腺炎的细菌——无乳链球菌、金黄色葡萄球菌、肺炎克雷伯菌和大肠杆菌进行了抗菌活性测试。总体而言，丁基羟基茴香醚和叔丁基对苯二酚表现出最大的抗菌活性。这些酚类化合物在250至500 ppm浓度下对所有四种测试细菌均具有杀菌作用。丁基羟基甲苯对革兰氏阳性菌具有杀菌作用，但对大肠菌群无效。在250 ppm浓度下，乙二胺四乙酸二钠对革兰氏阳性菌具有杀菌作用，但对革兰氏阴性菌的效果要差得多。然而，二乙烯三胺五乙酸对革兰氏阴性菌，尤其是对大肠杆菌的生长抑制作用比乙二胺四乙酸更强。所有其他化合物对所有四种微生物的效果通常要差得多或无效。因此，丁基羟基茴香醚、丁基羟基甲苯、叔丁基对苯二酚、乙二胺四乙酸和二乙烯三胺五乙酸在预防或治疗牛乳腺炎方面可能具有实际意义。
