Southern Federal University, 194/2 Stachki Avenue, Rostov-on-Don 344090, Russia.
Southern Federal University, 194/2 Stachki Avenue, Rostov-on-Don 344090, Russia.
Microbiol Res. 2024 Dec;289:127920. doi: 10.1016/j.micres.2024.127920. Epub 2024 Sep 26.
Studying Rhodococcus erythropolis stress response is of significant scientific interest, since this microorganism is widely used for bioremediation of oil-contaminated sites and is essential for environmental biotechnology. In addition, much less data was published on molecular mechanisms of stress resistance and adaptation to effects of pollutants for Gram-positive oil degraders compared to Gram-negative ones. This study provided an assessment of changes in the transcription level of the soxR, sodA, sodC, oxyR, katE, katG, recA, dinB, sigF, sigH genes in the presence of decane, hexadecane, cyclohexane, benzene, naphthalene, anthracene and diesel fuel. Judging by the changes in the expression of target genes, hydrocarbons as the main carbon source caused oxidative stress in R. erythropolis cells, which resulted in DNA damage. It was documented by enhanced transcription of genes encoding antioxidant enzymes (superoxide dismutase and catalase), SOS response, DNA polymerase IV, and sigma factors of RNA polymerase SigH and SigF. At this, it was likely that in the presence of hydrocarbons, transcription of catalase genes (katE and katG) was coordinated primarily by the sigF regulator.
研究红球菌应激反应具有重要的科学意义，因为这种微生物被广泛用于生物修复受污染的地点，是环境生物技术的关键。此外，与革兰氏阴性菌相比，关于革兰氏阳性油降解菌的应激抵抗和适应污染物影响的分子机制的数据要少得多。本研究评估了在癸烷、十六烷、环己烷、苯、萘、蒽和柴油燃料存在的情况下，soxR、sodA、sodC、oxyR、katE、katG、recA、dinB、sigF、sigH 基因转录水平的变化。从目标基因表达的变化来看，碳氢化合物作为主要碳源导致红球菌细胞产生氧化应激，从而导致 DNA 损伤。这是通过编码抗氧化酶（超氧化物歧化酶和过氧化氢酶）、SOS 反应、DNA 聚合酶 IV 以及 RNA 聚合酶 SigH 和 SigF 的σ因子的转录增强来证明的。在这种情况下，在存在碳氢化合物的情况下，过氧化氢酶基因（katE 和 katG）的转录可能主要由 sigF 调节剂协调。
